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Open Access
Peer-reviewed
Research Article
The normal human lymph node cell classification and landscape defined by high-dimensional spatial proteomics
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Maddalena M. Bolognesi ,
Contributed equally to this work with: Maddalena M. Bolognesi, Lorenzo Dall’Olio, Gastone Castellani
Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Software, Visualization, Writing – original draft
Affiliations Istituto di Bioimmagini e Sistemi Biologici Complessi (IBSBC) – CNR Via F.lli Cervi, Segrate, Italy, NBFC, National Biodiversity Future Center, Palermo, Italy
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Lorenzo Dall’Olio ,
Contributed equally to this work with: Maddalena M. Bolognesi, Lorenzo Dall’Olio, Gastone Castellani
Roles Conceptualization, Formal analysis, Investigation, Methodology, Software, Visualization, Writing – original draft
Affiliation Laboratorio di Data Science and Bioinformatics, IRCCS Istituto delle Scienze Neurologiche di Bologna – AUSL BO Ospedale Bellaria, Bologna, Italy
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Giulio Eugenio Mandelli,
Roles Formal analysis, Resources, Validation, Writing – original draft
Affiliations Pathology Unit, Department of Molecular and Translational Medicine-DMMT, University of Brescia, Brescia, Italy, Pathology Unit, ASST Spedali Civili Di Brescia, Brescia, Italy
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Luisa Lorenzi,
Roles Data curation, Investigation, Resources
Affiliations Pathology Unit, Department of Molecular and Translational Medicine-DMMT, University of Brescia, Brescia, Italy, Pathology Unit, ASST Spedali Civili Di Brescia, Brescia, Italy
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Francesca M. Bosisio,
Roles Funding acquisition, Resources, Writing – original draft
Affiliations The Leuven Institute for Single-cell Omics (LISCO), KULeuven, Leuven, Belgium, Translational Cell and Tissue Research Unit, Department of imaging and pathology, KULeuven, Leuven, Belgium, Pathology Department, University Hospital of Leuven, Leuven, Belgium
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Ann M. Haberman,
Roles Investigation, Resources, Writing – original draft
Affiliations Department of Immunobiology, Yale University, New Haven, Connecticut, United States of America, Department of Laboratory Medicine, Yale University, New Haven, Connecticut, United States of America
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Govind Bhagat,
Roles Resources, Writing – original draft
Current address: Pathology, Saint Louis University School of Medicine, Saint Louis, MO, USA
Affiliation Pathology, Columbia University Irving Medical Center and New York Presbyterian Hospital, NewYork, New York, United States of America
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Simone Borghesi,
Roles Formal analysis, Methodology, Software, Writing – original draft
Affiliation Department of Mathematics and Applications, University of Milano Bicocca, Milan, Italy
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Mario Faretta,
Roles Data curation, Investigation, Methodology, Software, Validation
Affiliation Department of Experimental Oncology, European Institute of Oncology IRCCS, Milan, Italy
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Gastone Castellani ,
Contributed equally to this work with: Maddalena M. Bolognesi, Lorenzo Dall’Olio, Gastone Castellani
Roles Funding acquisition, Methodology, Supervision
Affiliation Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Bologna, Italy
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Giorgio Cattoretti
Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Writing – original draft, Writing – review & editing
* E-mail: giorgio.cattoretti@unimib.it
Affiliation Pathology, Department of Medicine and Surgery, Universitá di Milano-Bicocca, Monza, Michigan, Italy
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The normal human lymph node cell classification and landscape defined by high-dimensional spatial proteomics
- Maddalena M. Bolognesi,
- Lorenzo Dall’Olio,
- Giulio Eugenio Mandelli,
- Luisa Lorenzi,
- Francesca M. Bosisio,
- Ann M. Haberman,
- Govind Bhagat,
- Simone Borghesi,
- Mario Faretta,
- Gastone Castellani
- Published: April 21, 2026
- https://doi.org/10.1371/journal.pone.0346693