Refining fibroblast-to-cardiomyocyte transdifferentiation protocols to explore emergent self-organization in cardiac cultures

Elena Turchaninova; Sofya Robustova; Sandaara Kovalenko; Vitalii Dzhabrailov; Aleria Dolgodvorova; Serafima Romanova; Dmitriy Zybin; Mikhail Popov; Alsu Miftakhova; Sheida Frolova; Mikhail Slotvitsky; Alexander Romanov; Konstantin Agladze; Valeriya Tsvelaya

doi:10.1371/journal.pone.0343415

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Open Access

Peer-reviewed

Research Article

Refining fibroblast-to-cardiomyocyte transdifferentiation protocols to explore emergent self-organization in cardiac cultures

Elena Turchaninova ,

Roles Formal analysis, Investigation, Visualization, Writing – original draft

* E-mail: turchaninova.ea@phystech.edu (ET), vts93@yandex.ru (VT)

Affiliations E. Meshalkin National Medical Research Center of the Ministry of Health of the Russian Federation, Novosibirsk, Russia, Moscow Center for Advanced Studies, Moscow, Russia

https://orcid.org/0009-0003-8165-2595

⨯
Sofya Robustova,

Roles Investigation, Visualization, Writing – original draft

Affiliation Moscow Center for Advanced Studies, Moscow, Russia
⨯
Sandaara Kovalenko,

Roles Investigation, Writing – original draft

Affiliations E. Meshalkin National Medical Research Center of the Ministry of Health of the Russian Federation, Novosibirsk, Russia, Moscow Center for Advanced Studies, Moscow, Russia, M.F. Vladimirsky Moscow Regional Clinical Research Institute, Moscow, Russia
⨯
Vitalii Dzhabrailov,

Roles Formal analysis, Investigation, Software, Visualization, Writing – original draft

Affiliations E. Meshalkin National Medical Research Center of the Ministry of Health of the Russian Federation, Novosibirsk, Russia, Moscow Center for Advanced Studies, Moscow, Russia
⨯
Aleria Dolgodvorova,

Roles Investigation

Affiliation Moscow Center for Advanced Studies, Moscow, Russia
⨯
Serafima Romanova,

Roles Investigation

Affiliation Moscow Center for Advanced Studies, Moscow, Russia
⨯
Dmitriy Zybin,

Roles Resources

Affiliation M.F. Vladimirsky Moscow Regional Clinical Research Institute, Moscow, Russia
⨯
Mikhail Popov,

Roles Resources

Affiliation M.F. Vladimirsky Moscow Regional Clinical Research Institute, Moscow, Russia
⨯
Alsu Miftakhova,

Roles Investigation

Affiliation Moscow Center for Advanced Studies, Moscow, Russia
⨯
Sheida Frolova,

Roles Data curation

Affiliation M.F. Vladimirsky Moscow Regional Clinical Research Institute, Moscow, Russia
⨯
Mikhail Slotvitsky,

Roles Investigation, Methodology, Software, Visualization

Affiliations E. Meshalkin National Medical Research Center of the Ministry of Health of the Russian Federation, Novosibirsk, Russia, Moscow Center for Advanced Studies, Moscow, Russia, M.F. Vladimirsky Moscow Regional Clinical Research Institute, Moscow, Russia
⨯
Alexander Romanov,

Roles Funding acquisition, Resources, Writing – review & editing

Affiliations E. Meshalkin National Medical Research Center of the Ministry of Health of the Russian Federation, Novosibirsk, Russia, Novosibirsk State Medical University, Novosibirsk, Russia
⨯
Konstantin Agladze,

Roles Conceptualization, Data curation, Resources, Supervision, Validation, Writing – review & editing

Affiliations Moscow Center for Advanced Studies, Moscow, Russia, M.F. Vladimirsky Moscow Regional Clinical Research Institute, Moscow, Russia

https://orcid.org/0000-0002-9258-436X

⨯
Valeriya Tsvelaya

Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Methodology, Project administration, Resources, Supervision, Validation, Writing – review & editing

* E-mail: turchaninova.ea@phystech.edu (ET), vts93@yandex.ru (VT)

Affiliations E. Meshalkin National Medical Research Center of the Ministry of Health of the Russian Federation, Novosibirsk, Russia, Moscow Center for Advanced Studies, Moscow, Russia, M.F. Vladimirsky Moscow Regional Clinical Research Institute, Moscow, Russia
⨯

Refining fibroblast-to-cardiomyocyte transdifferentiation protocols to explore emergent self-organization in cardiac cultures

Elena Turchaninova,
Sofya Robustova,
Sandaara Kovalenko,
Vitalii Dzhabrailov,
Aleria Dolgodvorova,
Serafima Romanova,
Dmitriy Zybin,
Mikhail Popov,
Alsu Miftakhova,
Sheida Frolova

Published: March 23, 2026
https://doi.org/10.1371/journal.pone.0343415

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Fibroblasts
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Transdifferentiation
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Cardiomyocytes
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Heart
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