Practical guidelines for producing non-replicating canine adenovirus vectors

Denis Omara; Christian Ndekezi; Susan Mugaba; Angella Nakyanzi; Henry Bukenya; Josephine Bwogi; Fortunate Natwijuka; Anne Kapaata; Frank Kato; Drake Byamukama; Mercy Lorna Ayebale; David Patrick Kateete; Jennifer Serwanga; Stephen Cose; Sande James Obondo; Pontiano Kaleebu; Sheila Nina Balinda

doi:10.1371/journal.pone.0341642

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Peer-reviewed

Practical guidelines for producing non-replicating canine adenovirus vectors

Denis Omara ,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing

* E-mail: omaradenis2@gmail.com

Affiliations Uganda Virus Research Institute (UVRI), Entebbe, Uganda, Department of Immunology and Molecular Biology, School of Biomedical Sciences, College of Health Sciences, Makerere University, Kampala, Uganda, Medical Research Council/Uganda Virus Research Institute & London School of Hygiene and Tropical Medicine (MRC/UVRI & LSHTM) Uganda Research Unit, Entebbe, Uganda

https://orcid.org/0000-0001-7936-1754

⨯
Christian Ndekezi,

Roles Conceptualization, Methodology, Writing – review & editing

Affiliations Uganda Virus Research Institute (UVRI), Entebbe, Uganda, Department of Immunology and Molecular Biology, School of Biomedical Sciences, College of Health Sciences, Makerere University, Kampala, Uganda, Medical Research Council/Uganda Virus Research Institute & London School of Hygiene and Tropical Medicine (MRC/UVRI & LSHTM) Uganda Research Unit, Entebbe, Uganda
⨯
Susan Mugaba,

Roles Methodology, Validation, Writing – review & editing

Affiliation Medical Research Council/Uganda Virus Research Institute & London School of Hygiene and Tropical Medicine (MRC/UVRI & LSHTM) Uganda Research Unit, Entebbe, Uganda
⨯
Angella Nakyanzi,

Roles Methodology, Writing – review & editing

Affiliation Uganda Virus Research Institute (UVRI), Entebbe, Uganda
⨯
Henry Bukenya,

Roles Methodology, Resources, Validation

Affiliation Uganda Virus Research Institute (UVRI), Entebbe, Uganda
⨯
Josephine Bwogi,

Roles Resources

Affiliation Uganda Virus Research Institute (UVRI), Entebbe, Uganda
⨯
Fortunate Natwijuka,

Roles Methodology

Affiliations Department of Immunology and Molecular Biology, School of Biomedical Sciences, College of Health Sciences, Makerere University, Kampala, Uganda, Medical Research Council/Uganda Virus Research Institute & London School of Hygiene and Tropical Medicine (MRC/UVRI & LSHTM) Uganda Research Unit, Entebbe, Uganda

https://orcid.org/0000-0001-7713-497X

⨯
Anne Kapaata,

Roles Methodology, Writing – review & editing

Affiliation Medical Research Council/Uganda Virus Research Institute & London School of Hygiene and Tropical Medicine (MRC/UVRI & LSHTM) Uganda Research Unit, Entebbe, Uganda
⨯
Frank Kato,

Roles Methodology, Writing – review & editing

Affiliations Department of Immunology and Molecular Biology, School of Biomedical Sciences, College of Health Sciences, Makerere University, Kampala, Uganda, Medical Research Council/Uganda Virus Research Institute & London School of Hygiene and Tropical Medicine (MRC/UVRI & LSHTM) Uganda Research Unit, Entebbe, Uganda
⨯
Drake Byamukama,

Roles Methodology

Affiliation Medical Research Council/Uganda Virus Research Institute & London School of Hygiene and Tropical Medicine (MRC/UVRI & LSHTM) Uganda Research Unit, Entebbe, Uganda
⨯
Mercy Lorna Ayebale,

Roles Methodology

Affiliation Medical Research Council/Uganda Virus Research Institute & London School of Hygiene and Tropical Medicine (MRC/UVRI & LSHTM) Uganda Research Unit, Entebbe, Uganda
⨯
David Patrick Kateete,

Roles Supervision

Affiliation Department of Immunology and Molecular Biology, School of Biomedical Sciences, College of Health Sciences, Makerere University, Kampala, Uganda
⨯
Jennifer Serwanga,

Roles Supervision, Writing – review & editing

Affiliations Uganda Virus Research Institute (UVRI), Entebbe, Uganda, Medical Research Council/Uganda Virus Research Institute & London School of Hygiene and Tropical Medicine (MRC/UVRI & LSHTM) Uganda Research Unit, Entebbe, Uganda

https://orcid.org/0000-0002-6672-3688

⨯
Stephen Cose,

Roles Supervision, Writing – review & editing

Affiliations Department of Immunology and Molecular Biology, School of Biomedical Sciences, College of Health Sciences, Makerere University, Kampala, Uganda, Medical Research Council/Uganda Virus Research Institute & London School of Hygiene and Tropical Medicine (MRC/UVRI & LSHTM) Uganda Research Unit, Entebbe, Uganda
⨯
Sande James Obondo,

Roles Supervision, Writing – review & editing

Affiliation Department of Immunology and Molecular Biology, School of Biomedical Sciences, College of Health Sciences, Makerere University, Kampala, Uganda
⨯
Pontiano Kaleebu,

Roles Project administration

Affiliations Uganda Virus Research Institute (UVRI), Entebbe, Uganda, Medical Research Council/Uganda Virus Research Institute & London School of Hygiene and Tropical Medicine (MRC/UVRI & LSHTM) Uganda Research Unit, Entebbe, Uganda
⨯
[ ... ],
Sheila Nina Balinda

Roles Conceptualization, Funding acquisition, Project administration, Resources, Supervision, Writing – review & editing

Affiliations Uganda Virus Research Institute (UVRI), Entebbe, Uganda, Medical Research Council/Uganda Virus Research Institute & London School of Hygiene and Tropical Medicine (MRC/UVRI & LSHTM) Uganda Research Unit, Entebbe, Uganda
⨯
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Practical guidelines for producing non-replicating canine adenovirus vectors

Denis Omara,
Christian Ndekezi,
Susan Mugaba,
Angella Nakyanzi,
Henry Bukenya,
Josephine Bwogi,
Fortunate Natwijuka,
Anne Kapaata,
Frank Kato,
Drake Byamukama

Published: May 20, 2026
https://doi.org/10.1371/journal.pone.0341642

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