Automated counting of prostate cell types with image processing and machine learning

Babak Kamali Doust Azad; Aryan Norouzzadeh Hakimi; Seyed Mohammad Tabatabaei; Bahar Mohammadi Arezouchi; Mohsen Mashhadi Keshtiban; Akram Mirzaei; Fatemeh Nafian; Fatemeh Khatami; Mohammad Kazem Aghamir; Mohammadreza Kolahdouz

doi:10.1371/journal.pone.0340628

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Open Access

Peer-reviewed

Research Article

Automated counting of prostate cell types with image processing and machine learning

Babak Kamali Doust Azad,

Roles Data curation, Formal analysis, Investigation, Project administration, Software, Supervision, Writing – original draft

Affiliation School of Electrical and Computer Engineering, College of Engineering, University of Tehran, Tehran, Iran
⨯
Aryan Norouzzadeh Hakimi,

Roles Data curation, Formal analysis, Software, Visualization, Writing – original draft

Affiliation Department of Biomedical Engineering, Tehran Islamic Azad University of Medical Sciences, Tehran, Iran
⨯
Seyed Mohammad Tabatabaei,

Roles Data curation, Formal analysis, Software, Visualization, Writing – original draft

Affiliation School of Electrical and Computer Engineering, College of Engineering, University of Tehran, Tehran, Iran
⨯
Bahar Mohammadi Arezouchi,

Roles Data curation, Formal analysis, Writing – original draft

Affiliation Department of Biomedical Engineering, Tehran Islamic Azad University of Medical Sciences, Tehran, Iran
⨯
Mohsen Mashhadi Keshtiban,

Roles Data curation, Formal analysis, Software, Validation, Visualization, Writing – original draft

Affiliation Department of Mechanical Engineering, Tarbiat Modares University, Tehran, Iran
⨯
Akram Mirzaei,

Roles Conceptualization, Writing – original draft, Writing – review & editing

Affiliation Urology Research Center, Tehran University of Medical Sciences, Tehran, Iran
⨯
Fatemeh Nafian,

Roles Methodology, Supervision

Affiliation Department of Medical Laboratory Sciences, TeMS.C., Islamic Azad University Tehran, Tehran, Iran
⨯
Fatemeh Khatami,

Roles Conceptualization, Resources, Supervision

Affiliation Urology Research Center, Tehran University of Medical Sciences, Tehran, Iran
⨯
Mohammad Kazem Aghamir,

Roles Conceptualization, Resources, Supervision

Affiliation Urology Research Center, Tehran University of Medical Sciences, Tehran, Iran
⨯
Mohammadreza Kolahdouz

Roles Conceptualization

* E-mail: kolahdouz@ut.ac.ir

Affiliation School of Electrical and Computer Engineering, College of Engineering, University of Tehran, Tehran, Iran

https://orcid.org/0000-0001-6992-6950

⨯

Automated counting of prostate cell types with image processing and machine learning

Babak Kamali Doust Azad,
Aryan Norouzzadeh Hakimi,
Seyed Mohammad Tabatabaei,
Bahar Mohammadi Arezouchi,
Mohsen Mashhadi Keshtiban,
Akram Mirzaei,
Fatemeh Nafian,
Fatemeh Khatami,
Mohammad Kazem Aghamir,
Mohammadreza Kolahdouz

Published: May 4, 2026
https://doi.org/10.1371/journal.pone.0340628

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Image processing
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Cell enumeration techniques
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Prostate cancer
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Hematocytometry
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DU145 cells
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