An immunofluorescence microscopy assay to discriminate distinct expression patterns of HIV-1 Gag and Nef proteins in HIV-1 provirus-harboring cells

Rosana Wiscovitch-Russo; Hongyan Sui; Mindy Smith; Hiromi Imamichi; H Clifford Lane; Tomozumi Imamichi

doi:10.1371/journal.pone.0340463

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Peer-reviewed

An immunofluorescence microscopy assay to discriminate distinct expression patterns of HIV-1 Gag and Nef proteins in HIV-1 provirus-harboring cells

Rosana Wiscovitch-Russo ,

Contributed equally to this work with: Rosana Wiscovitch-Russo, Hongyan Sui

Roles Data curation, Investigation, Validation, Writing – original draft

Affiliation Laboratory of Human Retrovirology and Immunoinformatics, Applied and Developmental Research Directorate, Frederick National Laboratory for Cancer Research, Frederick, Maryland, United States of America
⨯
Hongyan Sui ,

Contributed equally to this work with: Rosana Wiscovitch-Russo, Hongyan Sui

Roles Data curation, Formal analysis, Investigation, Methodology, Project administration, Supervision, Validation, Writing – original draft, Writing – review & editing

* E-mail: hongyan.sui@nih.gov

Affiliation Laboratory of Human Retrovirology and Immunoinformatics, Applied and Developmental Research Directorate, Frederick National Laboratory for Cancer Research, Frederick, Maryland, United States of America

https://orcid.org/0000-0002-4341-0929

⨯
Mindy Smith,

Roles Resources

Affiliation Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
⨯
Hiromi Imamichi,

Roles Conceptualization, Project administration, Resources, Writing – review & editing

Affiliation Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
⨯
H Clifford Lane,

Roles Conceptualization, Project administration, Supervision, Writing – review & editing

Affiliation Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
⨯
Tomozumi Imamichi

Roles Conceptualization, Funding acquisition, Project administration, Supervision, Writing – review & editing

Affiliation Laboratory of Human Retrovirology and Immunoinformatics, Applied and Developmental Research Directorate, Frederick National Laboratory for Cancer Research, Frederick, Maryland, United States of America
⨯

An immunofluorescence microscopy assay to discriminate distinct expression patterns of HIV-1 Gag and Nef proteins in HIV-1 provirus-harboring cells

Rosana Wiscovitch-Russo,
Hongyan Sui,
Mindy Smith,
Hiromi Imamichi,
H Clifford Lane,
Tomozumi Imamichi

Published: January 16, 2026
https://doi.org/10.1371/journal.pone.0340463

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Membrane proteins
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HIV-1
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Cloning
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