Highly efficient production of transgenic rats with long DNA insertions using piggyBac transposase mRNA and piezo-assisted microinjection

• Kohtaro Morita ,

  Roles Conceptualization, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Validation, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: kohtaro.morita@riken.jp

  Affiliations Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Kyoto, Japan, RIKEN BioResource Research Center, Tsukuba, Ibaraki, Japan

  https://orcid.org/0000-0003-2482-8155

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• Shunya Ihashi,

  Roles Investigation, Writing – review & editing

  Affiliation Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Kyoto, Japan

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• Eiichi Okamura,

  Roles Conceptualization, Resources, Writing – review & editing

  Affiliation Department of Stem Cells and Human Disease Models, Research Center for Animal Life Science, Shiga University of Medical Science, Otsu, Shiga, Japan

  https://orcid.org/0000-0002-1713-9290

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• Kazuya Goto,

  Roles Investigation, Writing – review & editing

  Affiliation Department of Regulation of Neurocognitive Disorders, Graduate School of Medicine, Kyoto University, Kyoto, Japan

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• Toru Yoshihara,

  Roles Conceptualization, Writing – review & editing

  Affiliation Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Kyoto, Japan

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• Arata Honda,

  Roles Conceptualization, Funding acquisition, Supervision, Writing – review & editing

  Affiliations RIKEN BioResource Research Center, Tsukuba, Ibaraki, Japan, Center for Development of Advanced Medical Technology, Jichi Medical University, Shimotsuke-shi, Tochigi, Japan

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• Masatsugu Ema,

  Roles Conceptualization, Funding acquisition, Resources, Supervision, Writing – review & editing

  Affiliation Department of Stem Cells and Human Disease Models, Research Center for Animal Life Science, Shiga University of Medical Science, Otsu, Shiga, Japan

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• Masahide Asano

  Roles Funding acquisition, Resources, Supervision, Writing – review & editing

  Affiliation Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Kyoto, Japan

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