Structural analysis of recombinant AAV vector genomes at single-molecule resolution

David Rouleau; Dimpal Lata; Serena Dollive; Robert E. Bruccoleri; Laura Van Lieshout; Diane Golebiowski; Ifeyinwa Iwuchukwu

doi:10.1371/journal.pone.0339201

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Open Access

Peer-reviewed

Research Article

Structural analysis of recombinant AAV vector genomes at single-molecule resolution

David Rouleau ,

Contributed equally to this work with: David Rouleau, Dimpal Lata

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Software, Validation, Visualization, Writing – original draft, Writing – review & editing

* E-mail: d.rouleau@oxb.com (DR); d.lata@oxb.com (DL)

Affiliation Oxford Biomedica (US) LLC, Bedford, Massachusetts, United States of America

https://orcid.org/0009-0008-9603-0605

⨯
Dimpal Lata ,

Contributed equally to this work with: David Rouleau, Dimpal Lata

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Software, Validation, Visualization, Writing – original draft, Writing – review & editing

* E-mail: d.rouleau@oxb.com (DR); d.lata@oxb.com (DL)

Affiliation Oxford Biomedica (US) LLC, Bedford, Massachusetts, United States of America

https://orcid.org/0000-0001-8556-7978

⨯
Serena Dollive,

Roles Project administration, Supervision, Writing – review & editing

Affiliation Oxford Biomedica (US) LLC, Bedford, Massachusetts, United States of America
⨯
Robert E. Bruccoleri,

Roles Conceptualization, Supervision, Writing – review & editing

Affiliations Oxford Biomedica (US) LLC, Bedford, Massachusetts, United States of America, Congenomics LLC, Glastonbury, Connecticut, United States of America

https://orcid.org/0009-0001-5687-4554

⨯
Laura Van Lieshout,

Roles Project administration, Supervision, Writing – review & editing

Affiliation Oxford Biomedica (US) LLC, Bedford, Massachusetts, United States of America
⨯
Diane Golebiowski,

Roles Supervision, Writing – review & editing

Affiliation Oxford Biomedica (US) LLC, Bedford, Massachusetts, United States of America

https://orcid.org/0000-0002-5550-7883

⨯
Ifeyinwa Iwuchukwu

Roles Supervision, Writing – review & editing

Affiliation Oxford Biomedica (US) LLC, Bedford, Massachusetts, United States of America
⨯

Structural analysis of recombinant AAV vector genomes at single-molecule resolution

David Rouleau,
Dimpal Lata,
Serena Dollive,
Robert E. Bruccoleri,
Laura Van Lieshout,
Diane Golebiowski,
Ifeyinwa Iwuchukwu

Published: July 30, 2026
https://doi.org/10.1371/journal.pone.0339201

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Genomics
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Sequence alignment
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RNA structure
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Genome analysis
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BLAST algorithm
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Structural genomics
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