Assessing fish diversity in the Amazon: The impact of primers and reference libraries in eDNA surveys

• Shizuka Hashimoto ,

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: shizuhashi@gmail.com (SH), tomas@evoamazon.net (TH)

  Affiliations Laboratório de Evolução e Genética Animal (LEGAL), Universidade Federal do Amazonas (UFAM), Manaus, Amazonas, Brazil, Programa de Pós-Graduação em Biologia de Água Doce e Pesca Interior (BADPI), Instituto Nacional de Pesquisas da Amazônia, Manaus, Amazonas, Brazil

  https://orcid.org/0000-0003-3188-1747

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• Sarah V. Carvalho,

  Roles Data curation, Investigation, Validation

  Affiliation Laboratório de Evolução e Genética Animal (LEGAL), Universidade Federal do Amazonas (UFAM), Manaus, Amazonas, Brazil

  https://orcid.org/0009-0003-0063-859X

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• Sonny de S. C. Miranda,

  Roles Formal analysis, Software

  Affiliation Instituto de Computação (IComp), Universidade Federal do Amazonas (UFAM), Manaus, Amazonas, Brazil

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• Valéria N. Machado,

  Roles Data curation, Investigation, Validation

  Affiliation Laboratório de Evolução e Genética Animal (LEGAL), Universidade Federal do Amazonas (UFAM), Manaus, Amazonas, Brazil

  https://orcid.org/0000-0003-4020-094X

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• Lucélia N. Carvalho,

  Roles Conceptualization, Funding acquisition, Project administration, Resources, Writing – original draft

  Affiliation Instituto de Ciências Naturais, Humanas e Sociais, Laboratório de Ictiologia Tropical (LIT), Universidade Federal de Mato Grosso-UFMT, Campus Universitário de Sinop, Mato Grosso, Brazil

  https://orcid.org/0000-0002-0673-0165

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• Cláudia P. de Deus,

  Roles Project administration, Supervision

  Affiliation Programa de Pós-Graduação em Biologia de Água Doce e Pesca Interior (BADPI), Instituto Nacional de Pesquisas da Amazônia, Manaus, Amazonas, Brazil

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• Izeni P. Farias,

  Roles Conceptualization, Funding acquisition, Project administration, Resources, Supervision, Writing – original draft, Writing – review & editing

  Affiliation Laboratório de Evolução e Genética Animal (LEGAL), Universidade Federal do Amazonas (UFAM), Manaus, Amazonas, Brazil

  https://orcid.org/0000-0002-1416-4351

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• Tomas Hrbek

  Roles Conceptualization, Formal analysis, Funding acquisition, Resources, Software, Supervision, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: shizuhashi@gmail.com (SH), tomas@evoamazon.net (TH)

  Affiliations Laboratório de Evolução e Genética Animal (LEGAL), Universidade Federal do Amazonas (UFAM), Manaus, Amazonas, Brazil, Department of Biology, Trinity University, San Antonio, Texas, United States of America

  https://orcid.org/0000-0003-3239-7068

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