Biochemical and molecular assessment of the superoxide dismutase (SOD) antioxidant enzyme regulation within Medicago truncatula root in response to iron deficiency

Nadia Kallala; Wissal M'sehli; Manel Chaouachi; Karima Jalali; Ghassen Abid; Haythem Mhadhbi

doi:10.1371/journal.pone.0335634

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Open Access

Peer-reviewed

Research Article

Biochemical and molecular assessment of the superoxide dismutase (SOD) antioxidant enzyme regulation within Medicago truncatula root in response to iron deficiency

Nadia Kallala ,

Roles Conceptualization, Data curation, Formal analysis, Methodology, Writing – original draft, Writing – review & editing

* E-mail: kallala.nadia@gmail.com, nadia.kallala@cbbc.rnrt.tn

Affiliation Laboratory of Legumes and Sustainable Agrosystems (L2AD), Centre of Biotechnology of Borj Cedria (CBBC) BP. 901, Hammam-Lif 2050, Tunisia

https://orcid.org/0009-0004-8031-4225

⨯
Wissal M'sehli,

Roles Conceptualization, Data curation, Methodology, Supervision, Writing – original draft, Writing – review & editing

Affiliation Laboratory of Legumes and Sustainable Agrosystems (L2AD), Centre of Biotechnology of Borj Cedria (CBBC) BP. 901, Hammam-Lif 2050, Tunisia
⨯
Manel Chaouachi,

Roles Data curation, Methodology

Affiliation Laboratory of Legumes and Sustainable Agrosystems (L2AD), Centre of Biotechnology of Borj Cedria (CBBC) BP. 901, Hammam-Lif 2050, Tunisia

https://orcid.org/0000-0001-7575-4817

⨯
Karima Jalali,

Roles Data curation, Formal analysis, Methodology

Affiliation Laboratory of Legumes and Sustainable Agrosystems (L2AD), Centre of Biotechnology of Borj Cedria (CBBC) BP. 901, Hammam-Lif 2050, Tunisia
⨯
Ghassen Abid,

Roles Conceptualization, Data curation, Methodology

Affiliation Laboratory of Legumes and Sustainable Agrosystems (L2AD), Centre of Biotechnology of Borj Cedria (CBBC) BP. 901, Hammam-Lif 2050, Tunisia
⨯
Haythem Mhadhbi

Roles Conceptualization, Data curation, Formal analysis, Methodology, Writing – original draft, Writing – review & editing

Affiliation Laboratory of Legumes and Sustainable Agrosystems (L2AD), Centre of Biotechnology of Borj Cedria (CBBC) BP. 901, Hammam-Lif 2050, Tunisia
⨯

Biochemical and molecular assessment of the superoxide dismutase (SOD) antioxidant enzyme regulation within Medicago truncatula root in response to iron deficiency

Nadia Kallala,
Wissal M'sehli,
Manel Chaouachi,
Karima Jalali,
Ghassen Abid,
Haythem Mhadhbi

Published: October 31, 2025
https://doi.org/10.1371/journal.pone.0335634

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Iron deficiency
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Superoxide dismutase
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Medicago truncatula
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Leaves
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Antioxidants
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Gene expression
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Bicarbonates
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Oxidative stress
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