A denaturation-free protocol for in situ visualization of short nuclear DNA sequences using padlock probes with rolling-circle amplification

• Ryoyo Ikebuchi ,

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: ryoyo.ikebuchi@igp.uu.se (RI); ulf.landegren@igp.uu.se (UL)

  Affiliation Molecular Tools, Department of Immunology, Genetics & Pathology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden

  https://orcid.org/0000-0003-0231-7733

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• Lu Xi,

  Roles Data curation, Writing – review & editing

  Affiliation Molecular Tools, Department of Immunology, Genetics & Pathology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden

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• Dimitra Bouri,

  Roles Investigation, Methodology, Validation

  Affiliation Molecular Tools, Department of Immunology, Genetics & Pathology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden

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• Valeriia Svintytska,

  Roles Investigation, Methodology, Validation

  Affiliation Molecular Tools, Department of Immunology, Genetics & Pathology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden

  https://orcid.org/0009-0004-3498-2425

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• Hanna Davies,

  Roles Investigation, Resources

  Affiliation Precision Medicine, Department of Immunology, Genetics & Pathology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden

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• Pawel Olszewski,

  Roles Investigation, Resources

  Affiliation Precision Medicine, Department of Immunology, Genetics & Pathology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden

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• Bozena Bruhn-Olszewska,

  Roles Investigation, Resources, Writing – review & editing

  Affiliation Precision Medicine, Department of Immunology, Genetics & Pathology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden

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• Jan P. Dumanski,

  Roles Funding acquisition, Project administration, Resources, Writing – review & editing

  Affiliation Precision Medicine, Department of Immunology, Genetics & Pathology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden

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• Ulf Landegren

  Roles Conceptualization, Funding acquisition, Project administration, Supervision, Writing – original draft, Writing – review & editing

  * E-mail: ryoyo.ikebuchi@igp.uu.se (RI); ulf.landegren@igp.uu.se (UL)

  Affiliation Molecular Tools, Department of Immunology, Genetics & Pathology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden

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