Functional expression of a Mo-dependent formate dehydrogenase in Escherichia coli under aerobic conditions

Marion Schulz; Anne Berger; David Roche; Emilie Pateau; Ivan Dubois; Valérie A. Delmas; Mélodie Cadillon; Madeleine Bouzon; Volker Döring

doi:10.1371/journal.pone.0334613

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Peer-reviewed

Research Article

Functional expression of a Mo-dependent formate dehydrogenase in Escherichia coli under aerobic conditions

Marion Schulz,

Roles Investigation, Methodology, Writing – original draft

Affiliation Génomique Métabolique, Genoscope, Institut François Jacob, CEA, CNRS, Univ Evry, Université Paris-Saclay, Evry-Courcouronnes, France
⨯
Anne Berger,

Roles Investigation

Affiliation Génomique Métabolique, Genoscope, Institut François Jacob, CEA, CNRS, Univ Evry, Université Paris-Saclay, Evry-Courcouronnes, France
⨯
David Roche,

Roles Formal analysis

Affiliation Génomique Métabolique, Genoscope, Institut François Jacob, CEA, CNRS, Univ Evry, Université Paris-Saclay, Evry-Courcouronnes, France
⨯
Emilie Pateau,

Roles Investigation

Affiliation Génomique Métabolique, Genoscope, Institut François Jacob, CEA, CNRS, Univ Evry, Université Paris-Saclay, Evry-Courcouronnes, France
⨯
Ivan Dubois,

Roles Investigation

Affiliation Génomique Métabolique, Genoscope, Institut François Jacob, CEA, CNRS, Univ Evry, Université Paris-Saclay, Evry-Courcouronnes, France
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Valérie A. Delmas,

Roles Investigation

Affiliation Génomique Métabolique, Genoscope, Institut François Jacob, CEA, CNRS, Univ Evry, Université Paris-Saclay, Evry-Courcouronnes, France
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Mélodie Cadillon,

Roles Investigation

Affiliation Génomique Métabolique, Genoscope, Institut François Jacob, CEA, CNRS, Univ Evry, Université Paris-Saclay, Evry-Courcouronnes, France
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Madeleine Bouzon,

Roles Conceptualization, Validation, Writing – original draft

Affiliation Génomique Métabolique, Genoscope, Institut François Jacob, CEA, CNRS, Univ Evry, Université Paris-Saclay, Evry-Courcouronnes, France
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Volker Döring

Roles Conceptualization, Supervision, Validation, Writing – original draft

* E-mail: vdoring@genoscope.cns.fr

Affiliation Génomique Métabolique, Genoscope, Institut François Jacob, CEA, CNRS, Univ Evry, Université Paris-Saclay, Evry-Courcouronnes, France

https://orcid.org/0000-0002-9453-6572

⨯

Functional expression of a Mo-dependent formate dehydrogenase in Escherichia coli under aerobic conditions

Marion Schulz,
Anne Berger,
David Roche,
Emilie Pateau,
Ivan Dubois,
Valérie A. Delmas,
Mélodie Cadillon,
Madeleine Bouzon,
Volker Döring

Published: October 29, 2025
https://doi.org/10.1371/journal.pone.0334613

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Formates
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Escherichia coli
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Necator
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Operons
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Pyruvate
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Plasmid construction
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Polymerase chain reaction
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