Efficient pathogen screening in honey bees: Application of FTA® cards for DNA storage and PCR analysis

Kristýna Myslínová; Silvie Dostálková; Jana Jemelková; Beata Hurychová; Ondřej Biemann; Jan Brus; Jana Fürstová; Marek Petřivalský; Jiří Danihlík

doi:10.1371/journal.pone.0334066

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Open Access

Peer-reviewed

Research Article

Efficient pathogen screening in honey bees: Application of FTA^® cards for DNA storage and PCR analysis

Kristýna Myslínová,

Roles Formal analysis, Investigation, Visualization, Writing – original draft

Affiliation Department of Biochemistry, Faculty of Science, Palacký University Olomouc, Olomouc, Czech Republic
⨯
Silvie Dostálková,

Roles Conceptualization, Writing – review & editing

Affiliation Department of Biochemistry, Faculty of Science, Palacký University Olomouc, Olomouc, Czech Republic
⨯
Jana Jemelková,

Roles Methodology, Writing – review & editing

Affiliation Department of Biochemistry, Faculty of Science, Palacký University Olomouc, Olomouc, Czech Republic
⨯
Beata Hurychová,

Roles Investigation

Affiliations Department of Biochemistry, Faculty of Science, Palacký University Olomouc, Olomouc, Czech Republic, Department of Cell Biology and Genetics, Faculty of Science, Palacký University Olomouc, Olomouc, Czech Republic

https://orcid.org/0009-0002-7021-156X

⨯
Ondřej Biemann,

Roles Visualization

Affiliation Department of Geoinformatics, Faculty of Science, Palacký University Olomouc, Olomouc, Czech Republic
⨯
Jan Brus,

Roles Visualization

Affiliation Department of Geoinformatics, Faculty of Science, Palacký University Olomouc, Olomouc, Czech Republic
⨯
Jana Fürstová,

Roles Formal analysis, Validation

Affiliation Olomouc University Social Health Institute (OUSHI), Palacký University Olomouc, Olomouc, Czech Republic
⨯
Marek Petřivalský,

Roles Supervision, Writing – review & editing

Affiliation Department of Biochemistry, Faculty of Science, Palacký University Olomouc, Olomouc, Czech Republic
⨯
Jiří Danihlík

Roles Conceptualization, Funding acquisition, Methodology, Project administration, Supervision, Writing – review & editing

* E-mail: jiri.danihlik@upol.cz

Affiliation Department of Biochemistry, Faculty of Science, Palacký University Olomouc, Olomouc, Czech Republic

https://orcid.org/0000-0002-6936-1766

⨯

Efficient pathogen screening in honey bees: Application of FTA^® cards for DNA storage and PCR analysis

Kristýna Myslínová,
Silvie Dostálková,
Jana Jemelková,
Beata Hurychová,
Ondřej Biemann,
Jan Brus,
Jana Fürstová,
Marek Petřivalský,
Jiří Danihlík

Published: October 30, 2025
https://doi.org/10.1371/journal.pone.0334066

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Pathogens
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Polymerase chain reaction
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Honey bees
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Spring
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Serratia marcescens
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DNA isolation
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Czech Republic
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