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Open Access
Peer-reviewed
Research Article
SARS-CoV-2 sequencing artifacts associated with targeted PCR enrichment and read mapping
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Kirsten Maren Ellegaard ,
Roles Conceptualization, Data curation, Formal analysis, Investigation, Software, Validation, Visualization, Writing – original draft, Writing – review & editing
* E-mail: krel@ssi.dk
Affiliation Department of Sequencing and Bioinformatics, Statens Serum Institut, Copenhagen, Denmark
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Vithiagaran Gunalan,
Roles Data curation, Investigation, Software, Validation, Writing – review & editing
Affiliation Department of Virology and Microbiological Preparedness, Statens Serum Institut, Copenhagen, Denmark
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Raphael Sieber,
Roles Data curation, Software, Validation, Writing – review & editing
Affiliation Department of Sequencing and Bioinformatics, Statens Serum Institut, Copenhagen, Denmark
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Sharmin Jamshid Baig,
Roles Investigation, Validation, Writing – review & editing
Affiliation Department of Sequencing and Bioinformatics, Statens Serum Institut, Copenhagen, Denmark
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Nicolai Balle Larsen,
Roles Investigation, Validation, Writing – review & editing
Affiliation Department of Virology and Microbiological Preparedness, Statens Serum Institut, Copenhagen, Denmark
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Marc Bennedbæk,
Roles Data curation, Software, Validation, Writing – review & editing
Affiliation Department of Virology and Microbiological Preparedness, Statens Serum Institut, Copenhagen, Denmark
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Jonas Bybjerg-Grauholm,
Roles Investigation, Validation, Writing – review & editing
Affiliation Department of Congenital Disorders, Statens Serum Institut, Copenhagen, Denmark
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Leandro Andrés Escobar-Herrera,
Roles Data curation, Software, Validation, Writing – review & editing
Affiliation Department of Sequencing and Bioinformatics, Statens Serum Institut, Copenhagen, Denmark
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Tobias Gress,
Roles Investigation, Validation, Writing – review & editing
Affiliation Department of Virology and Microbiological Preparedness, Statens Serum Institut, Copenhagen, Denmark
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Theis Hass Thorsen,
Roles Investigation, Validation, Writing – review & editing
Affiliation Department of Sequencing and Bioinformatics, Statens Serum Institut, Copenhagen, Denmark
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Anders Krusager,
Roles Investigation, Validation, Writing – review & editing
Affiliation Department of Virology and Microbiological Preparedness, Statens Serum Institut, Copenhagen, Denmark
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Gitte Nygaard Aasbjerg,
Roles Data curation, Software, Validation, Writing – review & editing
Affiliation Department of Sequencing and Bioinformatics, Statens Serum Institut, Copenhagen, Denmark
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Nour Saad Al-Tamimi,
Roles Investigation, Writing – review & editing
Affiliation Department of Congenital Disorders, Statens Serum Institut, Copenhagen, Denmark
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Casper Westergaard,
Roles Data curation, Software, Validation, Writing – review & editing
Affiliation Department of Sequencing and Bioinformatics, Statens Serum Institut, Copenhagen, Denmark
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Christina Wiid Svarrer,
Roles Investigation, Validation, Writing – review & editing
Affiliation Department 318 CMC Bioanalysis, Novo Nordisk, Copenhagen, Denmark
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Morten Rasmussen,
Roles Writing – review & editing
Affiliation Department of Virology and Microbiological Preparedness, Statens Serum Institut, Copenhagen, Denmark
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Marc Stegger
Roles Writing – original draft, Writing – review & editing
Affiliations Department of Sequencing and Bioinformatics, Statens Serum Institut, Copenhagen, Denmark, Antimicrobial Resistance and Infectious Diseases Laboratory, Harry Butler Institute, Murdoch University, Perth, Australia
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SARS-CoV-2 sequencing artifacts associated with targeted PCR enrichment and read mapping
- Kirsten Maren Ellegaard,
- Vithiagaran Gunalan,
- Raphael Sieber,
- Sharmin Jamshid Baig,
- Nicolai Balle Larsen,
- Marc Bennedbæk,
- Jonas Bybjerg-Grauholm,
- Leandro Andrés Escobar-Herrera,
- Tobias Gress,
- Theis Hass Thorsen
- Published: October 16, 2025
- https://doi.org/10.1371/journal.pone.0334009