Understanding and mitigating the impact of ambient mRNA contamination in single-cell RNA-sequencing analysis

• Jantarika Kumar Arora ,

  Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Software, Supervision, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: j.kumararora@qmul.ac.uk (JKA); varodom.cha@mahidol.ac.th (VC)

  Affiliations Centre for Immunobiology, Faculty of Medicine and Dentistry, Blizard Institute, Queen Mary University of London, London, England, Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand

  https://orcid.org/0000-0002-8025-0557

  ⨯
• Louisa K. James,

  Roles Funding acquisition, Investigation, Resources, Supervision, Writing – review & editing

  Affiliation Centre for Immunobiology, Faculty of Medicine and Dentistry, Blizard Institute, Queen Mary University of London, London, England

  https://orcid.org/0000-0002-2252-4636

  ⨯
• Varodom Charoensawan

  Roles Conceptualization, Funding acquisition, Investigation, Methodology, Supervision, Writing – original draft, Writing – review & editing

  * E-mail: j.kumararora@qmul.ac.uk (JKA); varodom.cha@mahidol.ac.th (VC)

  Affiliations Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand, Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand, Division of Medical Bioinformatics, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand, Siriraj Genomics, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand, Integrative Computational BioScience (ICBS) Center, Mahidol University, Nakhon Pathom, Thailand, School of Chemistry, Institute of Science, Suranaree University of Technology, Nakhon, Ratchasima, Thailand

  ⨯