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Open Access
Peer-reviewed
Research Article
A pipeline for rapid, high-throughput imaging and quantitative analysis of human intestinal organoids
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Faith M. Sawyer,
Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing – original draft
Affiliation Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, United States of America
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Fabio Stossi,
Roles Formal analysis, Methodology, Writing – review & editing
Current affiliation: Department of Chemical Biology and Therapeutics, High-Throughput Bioscience Center, St. Jude Children’s Research Hospital, Memphis, Tennessee, United States of America
Affiliations Integrated Microscopy Core, Baylor College of Medicine, Houston, Texas, United States of America, Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, United States of America
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Erika Nachman,
Roles Resources, Writing – review & editing
Affiliation Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, United States of America
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Robert A. Britton,
Roles Funding acquisition, Resources, Writing – review & editing
Affiliations Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, United States of America, Alkek Center for Metagenomics and Microbiome Research, Baylor College of Medicine, Houston, Texas, United States of America, Dan L. Duncan Comprehensive Cancer Research Center, Baylor College of Medicine, Houston, Texas, United States of America
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Michael J. Bolt,
Roles Data curation, Formal analysis, Investigation, Methodology, Resources, Visualization, Writing – review & editing
Affiliation Integrated Microscopy Core, Baylor College of Medicine, Houston, Texas, United States of America
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Michael A. Mancini,
Roles Conceptualization, Funding acquisition, Methodology, Supervision, Writing – review & editing
Affiliation Integrated Microscopy Core, Baylor College of Medicine, Houston, Texas, United States of America
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Mary K. Estes,
Roles Funding acquisition, Supervision, Writing – review & editing
Affiliations Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, United States of America, Department of Medicine, Baylor College of Medicine, Houston, Texas, United States of America
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Sarah E. Blutt
Roles Conceptualization, Funding acquisition, Methodology, Supervision, Writing – review & editing
* E-mail: sb691007@bcm.edu
Affiliations Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, United States of America, Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, United States of America
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A pipeline for rapid, high-throughput imaging and quantitative analysis of human intestinal organoids
- Faith M. Sawyer,
- Fabio Stossi,
- Erika Nachman,
- Robert A. Britton,
- Michael J. Bolt,
- Michael A. Mancini,
- Mary K. Estes,
- Sarah E. Blutt
- Published: October 8, 2025
- https://doi.org/10.1371/journal.pone.0332418