Direct real-time RT-PCR for the detection of dengue virus from patient serum in Lao PDR

Vilayouth Phimolsannousith; Malavanh Vongsouvath; Padthana Kiedsathid; Manivanh Vongsouvath; Elizabeth A. Ashley; Audrey Dubot-Pérès

doi:10.1371/journal.pone.0330459

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Open Access

Peer-reviewed

Research Article

Direct real-time RT-PCR for the detection of dengue virus from patient serum in Lao PDR

Vilayouth Phimolsannousith,

Roles Formal analysis, Investigation, Writing – original draft, Writing – review & editing

Affiliation Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Laos PDR
⨯
Malavanh Vongsouvath,

Roles Investigation

Affiliation Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Laos PDR
⨯
Padthana Kiedsathid,

Roles Investigation

Affiliation Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Laos PDR
⨯
Manivanh Vongsouvath,

Roles Resources, Supervision

Affiliation Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Laos PDR
⨯
Elizabeth A. Ashley,

Roles Funding acquisition, Project administration, Resources, Supervision, Writing – review & editing

Affiliations Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Laos PDR, Centre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom

https://orcid.org/0000-0002-7620-4822

⨯
Audrey Dubot-Pérès

Roles Conceptualization, Data curation, Formal analysis, Resources, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

* E-mail: audrey@tropmedres.ac

Affiliations Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Laos PDR, Centre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom, Unite des Virus Emergents (UVE: Aix-Marseille Univ, Universita di Corsica, IRD 190, Inserm, IRBA), Corsica, Marseille, France

https://orcid.org/0000-0003-1267-0725

⨯

Direct real-time RT-PCR for the detection of dengue virus from patient serum in Lao PDR

Vilayouth Phimolsannousith,
Malavanh Vongsouvath,
Padthana Kiedsathid,
Manivanh Vongsouvath,
Elizabeth A. Ashley,
Audrey Dubot-Pérès

Published: August 18, 2025
https://doi.org/10.1371/journal.pone.0330459

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RNA purification
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Dengue fever
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Lao people
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RNA extraction
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