Improved protocol for the vitrification and warming of rat zygotes by optimizing the warming solution and oocyte donor age

• Naomi Nakagata ,

  Contributed equally to this work with: Naomi Nakagata, Satohiro Nakao

  Roles Funding acquisition, Investigation, Methodology, Writing – original draft

  * E-mail: nakagata@kumamoto-u.ac.jp

  Affiliation Division of Reproductive Biotechnology and Innovation, Center for Animal Resources and Development, Institute of Resource Development and Analysis, Kumamoto University, Kumamoto, Japan

  https://orcid.org/0000-0002-5737-9993

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• Satohiro Nakao ,

  Contributed equally to this work with: Naomi Nakagata, Satohiro Nakao

  Roles Data curation, Investigation, Validation, Writing – original draft

  Affiliation Division of Reproductive Engineering, Center for Animal Resources and Development, Institute of Resource Development and Analysis, Kumamoto University, Kumamoto, Japan

  ⨯
• Nobuyuki Mikoda,

  Roles Data curation

  Affiliations Division of Reproductive Biotechnology and Innovation, Center for Animal Resources and Development, Institute of Resource Development and Analysis, Kumamoto University, Kumamoto, Japan, Kyudo Co., Ltd., Saga, Japan

  ⨯
• Katsuma Yamaga,

  Roles Investigation

  Affiliation Division of Reproductive Engineering, Center for Animal Resources and Development, Institute of Resource Development and Analysis, Kumamoto University, Kumamoto, Japan

  ⨯
• Yoshiko Nakagawa,

  Roles Investigation

  Affiliation Division of Reproductive Engineering, Center for Animal Resources and Development, Institute of Resource Development and Analysis, Kumamoto University, Kumamoto, Japan

  ⨯
• Tetsushi Sakuma,

  Roles Investigation

  Affiliation Laboratory of Genome-Editing Breeding, Graduate School of Agriculture, Kyoto University, Kyoto, Japan

  https://orcid.org/0000-0003-0396-1563

  ⨯
• Takashi Yamamoto,

  Roles Investigation

  Affiliations Genome Editing Innovation Center, Hiroshima University, Higashi‐Hiroshima, Japan, Division of Integrated Sciences for Life, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi‐Hiroshima, Japan

  ⨯
• Toru Takeo

  Roles Writing – review & editing

  Affiliation Division of Reproductive Engineering, Center for Animal Resources and Development, Institute of Resource Development and Analysis, Kumamoto University, Kumamoto, Japan

  https://orcid.org/0000-0002-8103-651X

  ⨯