Methodological establishment and diagnostic value of a multiplex fluorescent PCR assay for the detection of three fastidious respiratory pathogens

Jingchao Shi; Yijun Zhu; Shuyun Chen; Xiaoyun Shan; Lihong Bo; Kai Shen; Keqiang Chen

doi:10.1371/journal.pone.0328651

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Open Access

Peer-reviewed

Research Article

Methodological establishment and diagnostic value of a multiplex fluorescent PCR assay for the detection of three fastidious respiratory pathogens

Jingchao Shi,

Roles Conceptualization, Data curation, Methodology, Software, Validation, Visualization, Writing – original draft, Writing – review & editing

Affiliation Department of Clinical Laboratory, Affiliated Jinhua Hospital, Zhejiang University School of Medicine (Jinhua Municipal Central Hospital), Jinhua, Zhejiang Province, China
⨯
Yijun Zhu ,

Roles Funding acquisition, Investigation, Methodology, Project administration

* E-mail: zhuyijunwz@sina.com

Affiliation Department of Clinical Laboratory, Affiliated Jinhua Hospital, Zhejiang University School of Medicine (Jinhua Municipal Central Hospital), Jinhua, Zhejiang Province, China

https://orcid.org/0000-0001-9585-1235

⨯
Shuyun Chen,

Roles Data curation, Software, Validation, Visualization

Affiliations Hangzhou Dian Medical Center, Hangzhou, Zhejiang Province, China, Key Laboratory of Digital Technology in Medical Diagnostics of Zhejiang Province, Hangzhou, Zhejiang Province, China
⨯
Xiaoyun Shan,

Roles Funding acquisition, Methodology, Resources

Affiliation Department of Clinical Laboratory, Affiliated Jinhua Hospital, Zhejiang University School of Medicine (Jinhua Municipal Central Hospital), Jinhua, Zhejiang Province, China
⨯
Lihong Bo,

Roles Methodology, Resources

Affiliation Department of Clinical Laboratory, Affiliated Jinhua Hospital, Zhejiang University School of Medicine (Jinhua Municipal Central Hospital), Jinhua, Zhejiang Province, China
⨯
Kai Shen,

Roles Validation, Visualization

Affiliation Department of Clinical Laboratory, Affiliated Jinhua Hospital, Zhejiang University School of Medicine (Jinhua Municipal Central Hospital), Jinhua, Zhejiang Province, China
⨯
Keqiang Chen

Roles Resources, Visualization

Affiliation Department of Clinical Laboratory, Affiliated Jinhua Hospital, Zhejiang University School of Medicine (Jinhua Municipal Central Hospital), Jinhua, Zhejiang Province, China
⨯

Methodological establishment and diagnostic value of a multiplex fluorescent PCR assay for the detection of three fastidious respiratory pathogens

Jingchao Shi,
Yijun Zhu,
Shuyun Chen,
Xiaoyun Shan,
Lihong Bo,
Kai Shen,
Keqiang Chen

Published: July 31, 2025
https://doi.org/10.1371/journal.pone.0328651

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Haemophilus influenzae
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