Nested PCR amplification of the mitochondrial hypervariable region for non-invasive eDNA detection of Cryptobranchus alleganiensis

• Patrick Kurtz,

  Roles Formal analysis, Investigation, Writing – original draft, Writing – review & editing

  Affiliation Department of Chemical and Environmental Engineering, University of Cincinnati, Cincinnati, Ohio, United States of America

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• Jorge Santo Domingo,

  Roles Writing – review & editing

  Affiliation United States Environmental Protection Agency, Cincinnati, Ohio, United States of America

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• Robert Alexander Pyron,

  Roles Resources, Writing – review & editing

  Affiliation Department of Biological Sciences, The George Washington University, Washington, DC, United States of America

  https://orcid.org/0000-0003-2524-1794

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• David Wendell

  Roles Formal analysis, Funding acquisition, Resources, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: wendeldw@ucmail.uc.edu

  Affiliation Department of Chemical and Environmental Engineering, University of Cincinnati, Cincinnati, Ohio, United States of America

  https://orcid.org/0009-0008-4906-4837

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