CRISPR-Cas9 targeting the bla_KPC gene in a clinical isolate of Klebsiella michiganensis: Reduction of imipenem resistance and changes in genomic carbapenem resistance determinants

• Thaysa Leite Tagliaferri ,

  Roles Conceptualization, Funding acquisition, Investigation, Writing – original draft, Writing – review & editing

  * E-mail: thaysa.tagliaferri@rwth-aachen.de

  Affiliations Institute of Medical Microbiology, RWTH Aachen University Hospital, Aachen, Germany, Department of Microbiology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil

  https://orcid.org/0000-0003-0612-4808

  ⨯
• Alex Krüttgen,

  Roles Investigation, Resources

  Affiliation Laboratory Diagnostic Center, RWTH Aachen University Hospital, Aachen, Germany

  https://orcid.org/0000-0002-7157-8454

  ⨯
• Tiago Antônio de Oliveira Mendes ,

  Roles Conceptualization, Funding acquisition, Supervision, Writing – original draft, Writing – review & editing

  ¶☯ These authors contributed equally to this work and share the last authorship.

  Affiliation Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Viçosa, Brazil

  ⨯
• Simone Gonçalves dos Santos ,

  Roles Conceptualization, Funding acquisition, Supervision, Writing – review & editing

  ¶☯ These authors contributed equally to this work and share the last authorship.

  Affiliation Department of Microbiology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil

  https://orcid.org/0000-0002-5406-5470

  ⨯
• Hans-Peter Horz

  Roles Conceptualization, Funding acquisition, Project administration, Supervision, Writing – original draft, Writing – review & editing

  ¶☯ These authors contributed equally to this work and share the last authorship.

  Affiliation Institute of Medical Microbiology, RWTH Aachen University Hospital, Aachen, Germany

  ⨯