Incorporation of iPSCs together with TERT-immortalized keratinocytes and fibroblasts into reconstructed human gingiva enhances phenotype of gingival epithelium

Lisa-Lee Brueske; Sanne Roffel; Stephanie Beekhuis-Hoekstra; Helga E. de Vries; Susan Gibbs

doi:10.1371/journal.pone.0327728

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Open Access

Peer-reviewed

Research Article

Incorporation of iPSCs together with TERT-immortalized keratinocytes and fibroblasts into reconstructed human gingiva enhances phenotype of gingival epithelium

Lisa-Lee Brueske,

Roles Conceptualization, Investigation, Methodology, Writing – original draft

Affiliations Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, The Netherlands, Department of Molecular Cell Biology and Immunology, Amsterdam UMC Location Vrije Universiteit Amsterdam, Amsterdam, The Netherlands
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Sanne Roffel,

Roles Investigation

Affiliations Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, The Netherlands, Department of Molecular Cell Biology and Immunology, Amsterdam UMC Location Vrije Universiteit Amsterdam, Amsterdam, The Netherlands
⨯
Stephanie Beekhuis-Hoekstra,

Roles Conceptualization, Methodology, Resources, Writing – review & editing

Affiliation Department of Molecular Cell Biology and Immunology, Amsterdam UMC Location Vrije Universiteit Amsterdam, Amsterdam, The Netherlands
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Helga E. de Vries,

Roles Funding acquisition, Supervision, Writing – review & editing

Affiliations Department of Molecular Cell Biology and Immunology, Amsterdam UMC Location Vrije Universiteit Amsterdam, Amsterdam, The Netherlands, Amsterdam Neuroscience, Amsterdam UMC, Amsterdam, The Netherlands
⨯
Susan Gibbs

Roles Resources, Supervision, Writing – original draft, Writing – review & editing, Funding acquisition, Methodology, Conceptualization

* E-mail: s.gibbs@amsterdamumc.nl

Affiliations Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, The Netherlands, Department of Molecular Cell Biology and Immunology, Amsterdam UMC Location Vrije Universiteit Amsterdam, Amsterdam, The Netherlands, Amsterdam Movement Sciences, Amsterdam UMC, Amsterdam, The Netherlands

https://orcid.org/0000-0002-3446-6138

⨯

Incorporation of iPSCs together with TERT-immortalized keratinocytes and fibroblasts into reconstructed human gingiva enhances phenotype of gingival epithelium

Lisa-Lee Brueske,
Sanne Roffel,
Stephanie Beekhuis-Hoekstra,
Helga E. de Vries,
Susan Gibbs

Published: July 21, 2025
https://doi.org/10.1371/journal.pone.0327728

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Epithelium
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Gels
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Induced pluripotent stem cells
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Keratinocytes
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Apoptosis
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Cell differentiation
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DAPI staining
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