Flow cytometry protocol for cell death analysis in glioblastoma organoids: A technical note

• Anna-Laura Potthoff ,

  Roles Conceptualization, Formal analysis, Investigation, Supervision, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: anna-laura.potthoff@ukbonn.de (A-LP); s4mehsie@uni-bonn.de (M-CH)

  ¶☯ shared first authorship

  Affiliations Department of Neurosurgery, University Hospital Bonn, Bonn, Germany, Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany, Institute of Neuropathology, University Hospital Bonn, Bonn, Germany

  https://orcid.org/0000-0002-5710-6557

  ⨯
• Meng-Chun Hsieh ,

  Roles Conceptualization, Investigation, Writing – original draft, Writing – review & editing

  * E-mail: anna-laura.potthoff@ukbonn.de (A-LP); s4mehsie@uni-bonn.de (M-CH)

  ¶☯ shared first authorship

  Affiliations Department of Neurosurgery, University Hospital Bonn, Bonn, Germany, Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany

  ⨯
• Ahmad Melhem,

  Roles Investigation, Writing – review & editing

  Affiliations Department of Neurosurgery, University Hospital Bonn, Bonn, Germany, Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany

  ⨯
• Susanna S. Ng,

  Roles Formal analysis, Investigation, Writing – review & editing

  Affiliation Institute of Experimental Oncology, University Hospital Bonn, Bonn, Germany

  ⨯
• Barbara E. F. Pregler,

  Roles Investigation, Writing – review & editing

  Affiliations Department of Neurosurgery, University Hospital Bonn, Bonn, Germany, Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany

  ⨯
• Annika Vieregge,

  Roles Investigation, Writing – review & editing

  Affiliations Department of Neurosurgery, University Hospital Bonn, Bonn, Germany, Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany

  ⨯
• Markus Raspe,

  Roles Investigation, Writing – review & editing

  Affiliations Department of Neurosurgery, University Hospital Bonn, Bonn, Germany, Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany

  ⨯
• Lea L. Friker,

  Roles Writing – review & editing

  Affiliations Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany, Institute of Neuropathology, University Hospital Bonn, Bonn, Germany, Institute of Experimental Oncology, University Hospital Bonn, Bonn, Germany

  ⨯
• Thomas Zeyen,

  Roles Writing – review & editing

  Affiliations Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany, Department of Neurooncology, Center for Neurology, University Hospital Bonn, Bonn, Germany

  ⨯
• Julian P. Layer,

  Roles Writing – review & editing

  Affiliations Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany, Institute of Experimental Oncology, University Hospital Bonn, Bonn, Germany, Department of Radiation Oncology, University Hospital Bonn, Bonn, Germany

  https://orcid.org/0000-0001-7692-7775

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• Andreas Dolf,

  Roles Formal analysis, Writing – review & editing

  Affiliation Flow Cytometry Core Facility, Medical Faculty, University of Bonn, Bonn, Germany

  ⨯
• Marieta I. Toma,

  Roles Writing – review & editing

  Affiliation Department of Pathology, University Hospital Bonn, Bonn, Germany

  ⨯
• Andreas Waha,

  Roles Writing – review & editing

  Affiliation Institute of Neuropathology, University Hospital Bonn, Bonn, Germany

  ⨯
• Torsten Pietsch,

  Roles Writing – review & editing

  Affiliation Institute of Neuropathology, University Hospital Bonn, Bonn, Germany

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• Mike-Andrew Westhoff,

  Roles Writing – review & editing

  Affiliation Department of Pediatrics and Adolescent Medicine, University Medical Center Ulm, Ulm, Germany

  ⨯
• Hartmut Vatter,

  Roles Writing – review & editing

  Affiliations Department of Neurosurgery, University Hospital Bonn, Bonn, Germany, Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany

  ⨯
• Michael Hölzel,

  Roles Writing – review & editing

  Affiliation Institute of Experimental Oncology, University Hospital Bonn, Bonn, Germany

  ⨯
• Ulrich Herrlinger,

  Roles Writing – review & editing

  Affiliations Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany, Department of Neurooncology, Center for Neurology, University Hospital Bonn, Bonn, Germany

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•  [ ... ],
• Matthias Schneider

  Roles Conceptualization, Supervision, Writing – original draft, Writing – review & editing

  Affiliations Department of Neurosurgery, University Hospital Bonn, Bonn, Germany, Brain Tumor Translational Research Group, University Hospital Bonn, Bonn, Germany

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