Host-pathogen protein interaction studies: quality control of cDNA libraries using nanopore sequencing

Cécile Schimmich; Mathilde Gondard; Gregory Caignard; José-Carlos Valle-Casuso; Damien Vitour; François Piumi

doi:10.1371/journal.pone.0324917

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Open Access

Peer-reviewed

Research Article

Host-pathogen protein interaction studies: quality control of cDNA libraries using nanopore sequencing

Cécile Schimmich,

Roles Conceptualization, Formal analysis, Investigation, Methodology, Visualization, Writing – original draft, Writing – review & editing

Affiliation Unité physiopathologie et épidémiologie des maladies équines (PhEED), Laboratoire de Santé Animale, ANSES, Goustranville, France

https://orcid.org/0000-0001-7725-4664

⨯
Mathilde Gondard,

Roles Conceptualization, Investigation, Methodology, Writing – review & editing

Affiliation UMR Virologie, INRAE, École nationale vétérinaire d’Alfort, Anses, Laboratoire de Santé animale, Université Paris-Est, Maisons-Alfort, France
⨯
Gregory Caignard,

Roles Writing – review & editing

Affiliation UMR Virologie, INRAE, École nationale vétérinaire d’Alfort, Anses, Laboratoire de Santé animale, Université Paris-Est, Maisons-Alfort, France
⨯
José-Carlos Valle-Casuso,

Roles Funding acquisition, Project administration, Supervision, Writing – review & editing

Affiliations Unité physiopathologie et épidémiologie des maladies équines (PhEED), Laboratoire de Santé Animale, ANSES, Goustranville, France, Mixed Technological Unit “Equine Health and Welfare – Organisation and Traceability of the Equine Industry” (UMT SABOT), Goustranville, France
⨯
Damien Vitour,

Roles Conceptualization, Funding acquisition, Project administration, Supervision, Writing – review & editing

Affiliation UMR Virologie, INRAE, École nationale vétérinaire d’Alfort, Anses, Laboratoire de Santé animale, Université Paris-Est, Maisons-Alfort, France
⨯
François Piumi

Roles Conceptualization, Formal analysis, Investigation, Methodology, Project administration, Supervision, Visualization, Writing – original draft, Writing – review & editing

* E-mail: francois.piumi@vet-alfort.fr

Affiliation UMR Virologie, INRAE, École nationale vétérinaire d’Alfort, Anses, Laboratoire de Santé animale, Université Paris-Est, Maisons-Alfort, France

https://orcid.org/0000-0001-6036-7662

⨯

Host-pathogen protein interaction studies: quality control of cDNA libraries using nanopore sequencing

Cécile Schimmich,
Mathilde Gondard,
Gregory Caignard,
José-Carlos Valle-Casuso,
Damien Vitour,
François Piumi

Published: May 30, 2025
https://doi.org/10.1371/journal.pone.0324917

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DNA libraries
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Sequence alignment
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Human genomics
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Gene ontologies
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Transcriptome analysis
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Pseudogenes
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Gene sequencing
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Genomics
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