Use of allele-specific qPCR and PCR-RFLP analysis for rapid detection of the SARS-CoV-2 variants in Tunisia: A cheap flexible approach adapted for developing countries

• Wajdi Ayadi ,

  Roles Conceptualization, Investigation, Writing – original draft

  * E-mail: wajdi.ayadi.cbs@gmail.com

  Affiliation Laboratory of Molecular Biotechnology of Eukaryotes, Center of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia

  https://orcid.org/0000-0001-9962-9957

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• Fahmi Smaoui,

  Roles Data curation, Formal analysis, Software, Writing – original draft

  Affiliation Laboratory of Microbiology, Research Laboratory for Microorganisms and Human Disease LR03SP03, Habib Bourguiba University-Hospital, Sfax, Tunisia

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• Saba Gargouri,

  Roles Methodology, Supervision

  Affiliations Laboratory of Microbiology, Research Laboratory for Microorganisms and Human Disease LR03SP03, Habib Bourguiba University-Hospital, Sfax, Tunisia, Faculty of Medicine, University of Sfax, Sfax, Tunisia

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• Sana Ferjani,

  Roles Data curation, Investigation, Software

  Affiliations Charles Nicolle Hospital, Laboratory of Microbiology, Tunis, Tunisia, University of Tunis El Manar, Faculty of Medicine of Tunis, Tunis, Tunisia

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• Zaineb Hamzaoui,

  Roles Data curation, Software

  Affiliation University of Tunis El Manar, Faculty of Medicine of Tunis, Tunis, Tunisia

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• Awatef Taktak,

  Roles Formal analysis, Software

  Affiliations Laboratory of Molecular Biotechnology of Eukaryotes, Center of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia, Laboratory of Microbiology, Research Laboratory for Microorganisms and Human Disease LR03SP03, Habib Bourguiba University-Hospital, Sfax, Tunisia

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• Amel Chtourou,

  Roles Supervision, Validation

  Affiliations Laboratory of Microbiology, Research Laboratory for Microorganisms and Human Disease LR03SP03, Habib Bourguiba University-Hospital, Sfax, Tunisia, Faculty of Medicine, University of Sfax, Sfax, Tunisia

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• Houda Skouri-Gargouri,

  Roles Supervision, Validation

  Affiliation Laboratory of Molecular Biotechnology of Eukaryotes, Center of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia

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• Azza Hadj Sassi,

  Roles Investigation, Methodology

  Affiliation Laboratory of Molecular Biotechnology of Eukaryotes, Center of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia

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• Mouna Ben Sassi,

  Roles Data curation, Investigation

  Affiliations National Center Chalbi belkahia of Pharmacovigilance of Tunis, Laboratory of Clinical Pharmacology, Tunis, Tunisia, University of Tunis El Manar, Faculty of Medicine of Tunis, Tunis, Tunisia

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• Sameh Trabelsi,

  Roles Data curation, Investigation

  Affiliations National Center Chalbi belkahia of Pharmacovigilance of Tunis, Laboratory of Clinical Pharmacology, Tunis, Tunisia, University of Tunis El Manar, Faculty of Medicine of Tunis, Tunis, Tunisia

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• Ali Gargouri,

  Roles Supervision, Writing – review & editing

  Affiliation Laboratory of Molecular Biotechnology of Eukaryotes, Center of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia

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• Ilhem Boutiba-Ben Boubaker,

  Roles Supervision, Validation, Writing – review & editing

  Affiliations Charles Nicolle Hospital, Laboratory of Microbiology, Tunis, Tunisia, University of Tunis El Manar, Faculty of Medicine of Tunis, Tunis, Tunisia

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• Héla Karray-Hakim,

  Roles Supervision, Writing – review & editing

  Affiliations Laboratory of Microbiology, Research Laboratory for Microorganisms and Human Disease LR03SP03, Habib Bourguiba University-Hospital, Sfax, Tunisia, Faculty of Medicine, University of Sfax, Sfax, Tunisia

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• Raja Mokdad-Gargouri,

  Roles Supervision, Writing – review & editing

  Affiliation Laboratory of Molecular Biotechnology of Eukaryotes, Center of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia

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•  [ ... ],
• Lamia Feki-Berrajah

  Roles Conceptualization, Supervision, Validation, Writing – review & editing

  Affiliations Laboratory of Microbiology, Research Laboratory for Microorganisms and Human Disease LR03SP03, Habib Bourguiba University-Hospital, Sfax, Tunisia, Faculty of Medicine, University of Sfax, Sfax, Tunisia

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