Fluorometric combination analysis of conformational changes in urea-induced unfolding of soybean protein isolates and their complexes with casein

Yangchao Gao; Yuchuan Li; Yifan Yang; Hongying He; Yanqiong Li; Shuhui Yu; Haiying Wang; Yuyu Zhang; He Liu

doi:10.1371/journal.pone.0319864

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Open Access

Peer-reviewed

Research Article

Fluorometric combination analysis of conformational changes in urea-induced unfolding of soybean protein isolates and their complexes with casein

Yangchao Gao,

Roles Conceptualization, Methodology, Writing – original draft

Affiliation Yunnan Characteristic Resource Plants Intelligent Agriculture Engineering Center, College of Agriculture and Life Science, Kunming University, Kunming, China
⨯
Yuchuan Li,

Roles Conceptualization, Funding acquisition, Supervision

Affiliation Yunnan Characteristic Resource Plants Intelligent Agriculture Engineering Center, College of Agriculture and Life Science, Kunming University, Kunming, China
⨯
Yifan Yang,

Roles Formal analysis, Methodology, Writing – original draft

Affiliation Yunnan Characteristic Resource Plants Intelligent Agriculture Engineering Center, College of Agriculture and Life Science, Kunming University, Kunming, China
⨯
Hongying He,

Roles Data curation, Methodology

Affiliation Yunnan Characteristic Resource Plants Intelligent Agriculture Engineering Center, College of Agriculture and Life Science, Kunming University, Kunming, China
⨯
Yanqiong Li,

Roles Data curation, Methodology, Supervision, Writing – review & editing

Affiliation Yunnan Characteristic Resource Plants Intelligent Agriculture Engineering Center, College of Agriculture and Life Science, Kunming University, Kunming, China
⨯
Shuhui Yu,

Roles Data curation, Formal analysis, Supervision, Writing – review & editing

Affiliation Yunnan Characteristic Resource Plants Intelligent Agriculture Engineering Center, College of Agriculture and Life Science, Kunming University, Kunming, China
⨯
Haiying Wang,

Roles Conceptualization, Methodology, Supervision, Writing – review & editing

Affiliation Yunnan Characteristic Resource Plants Intelligent Agriculture Engineering Center, College of Agriculture and Life Science, Kunming University, Kunming, China
⨯
Yuyu Zhang,

Roles Funding acquisition, Investigation, Supervision, Writing – review & editing

Affiliation Yunnan Characteristic Resource Plants Intelligent Agriculture Engineering Center, College of Agriculture and Life Science, Kunming University, Kunming, China
⨯
He Liu

Roles Funding acquisition, Supervision

* E-mail: britney198601@163.com

Affiliation Yunnan Characteristic Resource Plants Intelligent Agriculture Engineering Center, College of Agriculture and Life Science, Kunming University, Kunming, China

https://orcid.org/0000-0002-2379-5446

⨯

Fluorometric combination analysis of conformational changes in urea-induced unfolding of soybean protein isolates and their complexes with casein

Yangchao Gao,
Yuchuan Li,
Yifan Yang,
Hongying He,
Yanqiong Li,
Shuhui Yu,
Haiying Wang,
Yuyu Zhang,
He Liu

Published: March 31, 2025
https://doi.org/10.1371/journal.pone.0319864

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Urea
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Casein
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Turbidity
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Fluorescence
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Globular proteins
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Phase diagrams
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Iodides
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Protein complexes
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