Systematic identification and characterization of high efficiency Cas9 guide RNAs for therapeutic targeting of ADAR

Benjamin G. Gowen; Kory Melton; Weng In Leong; Prachi Khekare; Shannon McCawley; Jean Chan; Pierre Boivin; Vihasi Jani; Aaron J. Cantor; Akshay Tambe; Mary Haak-Frendscho; Mary J. Janatpour; Spencer C. Wei

doi:10.1371/journal.pone.0317745

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Open Access

Peer-reviewed

Research Article

Systematic identification and characterization of high efficiency Cas9 guide RNAs for therapeutic targeting of ADAR

Benjamin G. Gowen ,

Roles Conceptualization, Supervision, Writing – original draft, Writing – review & editing

* E-mail: benjamin.gowen@gmail.com (BGG); spencer.wei@gmail.com (SCW)

¶☯ Authors contributed equally.

Affiliation Spotlight Therapeutics, Hayward, California, United States of America

https://orcid.org/0000-0002-1111-3284

⨯
Kory Melton ,

Contributed equally to this work with: Kory Melton

Roles Data curation, Formal analysis, Investigation, Methodology, Software, Validation, Visualization, Writing – original draft, Writing – review & editing

¶☯ Authors contributed equally.

Affiliation Spotlight Therapeutics, Hayward, California, United States of America
⨯
Weng In Leong,

Roles Investigation, Methodology, Supervision, Validation, Visualization

Affiliation Spotlight Therapeutics, Hayward, California, United States of America
⨯
Prachi Khekare,

Roles Data curation, Formal analysis, Investigation, Methodology, Software, Validation, Visualization

Affiliation Spotlight Therapeutics, Hayward, California, United States of America
⨯
Shannon McCawley,

Roles Formal analysis, Investigation, Methodology, Validation, Visualization

Affiliation Spotlight Therapeutics, Hayward, California, United States of America
⨯
Jean Chan,

Roles Formal analysis, Investigation, Methodology, Validation, Visualization

Affiliation Spotlight Therapeutics, Hayward, California, United States of America
⨯
Pierre Boivin,

Roles Formal analysis, Investigation, Methodology, Validation, Visualization

Affiliation Spotlight Therapeutics, Hayward, California, United States of America
⨯
Vihasi Jani,

Roles Formal analysis, Investigation, Methodology, Validation, Visualization

Affiliation Spotlight Therapeutics, Hayward, California, United States of America
⨯
Aaron J. Cantor,

Roles Supervision, Writing – review & editing

Affiliation Spotlight Therapeutics, Hayward, California, United States of America

https://orcid.org/0000-0002-2158-6253

⨯
Akshay Tambe,

Roles Data curation, Software, Supervision

Affiliation Spotlight Therapeutics, Hayward, California, United States of America
⨯
Mary Haak-Frendscho,

Roles Funding acquisition, Project administration, Writing – review & editing

Affiliation Spotlight Therapeutics, Hayward, California, United States of America
⨯
Mary J. Janatpour,

Roles Conceptualization, Funding acquisition, Project administration, Writing – review & editing

Affiliation Spotlight Therapeutics, Hayward, California, United States of America
⨯
Spencer C. Wei

Roles Conceptualization, Supervision, Writing – original draft, Writing – review & editing

* E-mail: benjamin.gowen@gmail.com (BGG); spencer.wei@gmail.com (SCW)

Affiliation Spotlight Therapeutics, Hayward, California, United States of America

https://orcid.org/0000-0001-9673-9683

⨯

Systematic identification and characterization of high efficiency Cas9 guide RNAs for therapeutic targeting of ADAR

Benjamin G. Gowen,
Kory Melton,
Weng In Leong,
Prachi Khekare,
Shannon McCawley,
Jean Chan,
Pierre Boivin,
Vihasi Jani,
Aaron J. Cantor,
Akshay Tambe

Published: February 24, 2025
https://doi.org/10.1371/journal.pone.0317745

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