A protocol for loading Calcein-AM into extracellular vesicles from mammalian cells for clear visualization with a fluorescence microscope coupled to a deconvolution system

• María-Angélica Calderón-Peláez,

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing

  Affiliation Virology Group, Vice-chancellor of Research, Universidad El Bosque, Bogotá, Colombia

  https://orcid.org/0000-0003-0788-0795

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• Jaime E. Castellanos,

  Roles Supervision, Writing – review & editing

  Affiliation Virology Group, Vice-chancellor of Research, Universidad El Bosque, Bogotá, Colombia

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• Myriam L. Velandia-Romero

  Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Methodology, Project administration, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: velandiamyriam@unbosque.edu.co

  Affiliation Virology Group, Vice-chancellor of Research, Universidad El Bosque, Bogotá, Colombia

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