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Open Access
Peer-reviewed
Research Article
Stem cell culture conditions affect in vitro differentiation potential and mouse gastruloid formation
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Marloes Blotenburg ,
Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing
* E-mail: peter.zeller@mbg.au.dk (PZ), marloes.blotenburg@unil.ch (MB)
Affiliations Hubrecht Institute-KNAW (Royal Netherlands Academy of Arts and Sciences), Oncode Institute, Utrecht, The Netherlands, University Medical Center Utrecht, Utrecht, The Netherlands
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Lianne Suurenbroek ,
Contributed equally to this work with: Lianne Suurenbroek, Danique Bax
Roles Data curation, Formal analysis, Investigation, Methodology, Validation, Writing – original draft, Writing – review & editing
Affiliations Hubrecht Institute-KNAW (Royal Netherlands Academy of Arts and Sciences), Oncode Institute, Utrecht, The Netherlands, University Medical Center Utrecht, Utrecht, The Netherlands
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Danique Bax ,
Contributed equally to this work with: Lianne Suurenbroek, Danique Bax
Roles Data curation, Formal analysis, Investigation, Methodology, Validation, Writing – review & editing
Affiliation Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences (RIMLS), Radboud University Nijmegen, Nijmegen, The Netherlands
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Joëlle de Visser,
Roles Data curation, Formal analysis, Investigation, Methodology, Validation
Affiliation Developmental, Stem Cell and Cancer Biology, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands
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Vivek Bhardwaj,
Roles Formal analysis
Affiliations Hubrecht Institute-KNAW (Royal Netherlands Academy of Arts and Sciences), Oncode Institute, Utrecht, The Netherlands, University Medical Center Utrecht, Utrecht, The Netherlands
⨯ - Luca Braccioli,
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Elzo de Wit,
Roles Resources, Supervision
Affiliation The Netherlands Cancer Institute, Amsterdam, The Netherlands
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Antonius van Boxtel,
Roles Supervision, Writing – review & editing
Affiliation Developmental, Stem Cell and Cancer Biology, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands
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Hendrik Marks,
Roles Funding acquisition, Project administration, Supervision, Validation, Writing – review & editing
Affiliation Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences (RIMLS), Radboud University Nijmegen, Nijmegen, The Netherlands
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Peter Zeller
Roles Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing
* E-mail: peter.zeller@mbg.au.dk (PZ), marloes.blotenburg@unil.ch (MB)
Affiliations Hubrecht Institute-KNAW (Royal Netherlands Academy of Arts and Sciences), Oncode Institute, Utrecht, The Netherlands, University Medical Center Utrecht, Utrecht, The Netherlands, Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark
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Stem cell culture conditions affect in vitro differentiation potential and mouse gastruloid formation
- Marloes Blotenburg,
- Lianne Suurenbroek,
- Danique Bax,
- Joëlle de Visser,
- Vivek Bhardwaj,
- Luca Braccioli,
- Elzo de Wit,
- Antonius van Boxtel,
- Hendrik Marks,
- Peter Zeller
- Published: March 26, 2025
- https://doi.org/10.1371/journal.pone.0317309