Surfaces environmental monitoring of SARS-CoV-2: Loop mediated isothermal amplification (LAMP) and droplet digital PCR (ddPCR) in comparison with standard Reverse-Transcription quantitative polymerase chain reaction (RT-qPCR) techniques

Simona Spiteri; Irene Salamon; Luna Girolamini; Maria Rosaria Pascale; Federica Marino; Carlo Derelitto; Laura Caligaris; Simone Paghera; Manuela Ferracin; Sandra Cristino

doi:10.1371/journal.pone.0317228

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Open Access

Peer-reviewed

Research Article

Surfaces environmental monitoring of SARS-CoV-2: Loop mediated isothermal amplification (LAMP) and droplet digital PCR (ddPCR) in comparison with standard Reverse-Transcription quantitative polymerase chain reaction (RT-qPCR) techniques

Simona Spiteri,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Software, Writing – original draft

Affiliations Department of Biological, Geological, and Environmental Sciences, University of Bologna, Bologna, Italy, Department of Medical and Surgical Sciences, University of Bologna, Bologna, Italy
⨯
Irene Salamon,

Roles Formal analysis, Methodology

Affiliation IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, Italy
⨯
Luna Girolamini,

Roles Formal analysis

Affiliation Department of Biological, Geological, and Environmental Sciences, University of Bologna, Bologna, Italy

https://orcid.org/0000-0001-5916-9634

⨯
Maria Rosaria Pascale,

Roles Formal analysis, Investigation, Writing – review & editing

Affiliation Department of Biological, Geological, and Environmental Sciences, University of Bologna, Bologna, Italy
⨯
Federica Marino,

Roles Formal analysis

Affiliation Department of Biological, Geological, and Environmental Sciences, University of Bologna, Bologna, Italy
⨯
Carlo Derelitto,

Roles Formal analysis

Affiliation Department of Biological, Geological, and Environmental Sciences, University of Bologna, Bologna, Italy
⨯
Laura Caligaris,

Roles Formal analysis

Affiliation Department of Biological, Geological, and Environmental Sciences, University of Bologna, Bologna, Italy
⨯
Simone Paghera,

Roles Formal analysis

Affiliation Copan Italia, Brescia, Italy
⨯
Manuela Ferracin,

Roles Conceptualization, Supervision

Affiliation Department of Medical and Surgical Sciences, University of Bologna, Bologna, Italy

https://orcid.org/0000-0002-1595-6887

⨯
Sandra Cristino

Roles Conceptualization, Data curation, Investigation, Methodology, Supervision, Writing – original draft

* E-mail: sandra.cristino@unibo.it

Affiliation Department of Biological, Geological, and Environmental Sciences, University of Bologna, Bologna, Italy

https://orcid.org/0000-0002-5084-2615

⨯

Surfaces environmental monitoring of SARS-CoV-2: Loop mediated isothermal amplification (LAMP) and droplet digital PCR (ddPCR) in comparison with standard Reverse-Transcription quantitative polymerase chain reaction (RT-qPCR) techniques

Simona Spiteri,
Irene Salamon,
Luna Girolamini,
Maria Rosaria Pascale,
Federica Marino,
Carlo Derelitto,
Laura Caligaris,
Simone Paghera,
Manuela Ferracin,
Sandra Cristino

Published: February 3, 2025
https://doi.org/10.1371/journal.pone.0317228

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