Optimizing RNA extraction methods for high-throughput transcriptome sequencing of formalin-fixed paraffin-embedded cardiac tissue specimens

• Nkechi Martina Odogwu ,

  Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Software, Validation, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: Nelson.Timothy@mayo.edu (TJN); Odogwu.Nkechi@mayo.edu (NMO)

  Affiliation Program for Hypoplastic Left Heart Syndrome, Mayo Clinic Rochester, Rochester, Minnesota, United States of America

  https://orcid.org/0000-0001-7824-0096

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• Jin Sung Jang,

  Roles Data curation, Formal analysis, Investigation, Methodology, Resources, Software, Writing – review & editing

  Affiliations Genome Analysis Core, Medical Genome Facility, Center for Individualized Medicine, Mayo Clinic Rochester, Rochester, Minnesota, United States of America, Department of Laboratory Medicine and Pathology, Mayo Clinic Rochester, Rochester, Minnesota, United States of America

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• Sabrina Albertson,

  Roles Data curation, Formal analysis, Methodology, Software, Validation, Visualization, Writing – review & editing

  Affiliation Program for Hypoplastic Left Heart Syndrome, Mayo Clinic Rochester, Rochester, Minnesota, United States of America

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• Clinton Hagen,

  Roles Data curation, Formal analysis, Investigation, Software, Supervision, Validation

  Affiliation Program for Hypoplastic Left Heart Syndrome, Mayo Clinic Rochester, Rochester, Minnesota, United States of America

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• Boyd Rasmussen,

  Roles Investigation, Methodology, Project administration, Resources, Software, Writing – review & editing

  Affiliation Program for Hypoplastic Left Heart Syndrome, Mayo Clinic Rochester, Rochester, Minnesota, United States of America

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• Oommen Saji,

  Roles Formal analysis, Investigation, Project administration, Resources, Software, Validation, Writing – review & editing

  Affiliation Program for Hypoplastic Left Heart Syndrome, Mayo Clinic Rochester, Rochester, Minnesota, United States of America

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• Timothy J. Nelson ,

  Roles Formal analysis, Funding acquisition, Project administration, Resources, Supervision, Validation, Writing – review & editing

  * E-mail: Nelson.Timothy@mayo.edu (TJN); Odogwu.Nkechi@mayo.edu (NMO)

  Affiliations Program for Hypoplastic Left Heart Syndrome, Mayo Clinic Rochester, Rochester, Minnesota, United States of America, Division of Cardiovascular Medicine, General Internal Medicine, Mayo Clinic Rochester, Rochester, Minnesota, United States of America, Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic Rochester, Rochester, Minnesota, United States of America, Center for Regenerative Medicine, Mayo Clinic Rochester, Rochester, Minnesota, United States of America

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• the Wanek Program Preclinical Pipeline

  ¶The Wanek Program Preclinical Pipeline are listed in the acknowledgments aspect of the article.

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