Development of an optimized protocol for generating knockout cancer cell lines using the CRISPR/Cas9 system, with emphasis on transient transfection

• Seyed Alireza Mousavi Kahaki,

  Roles Formal analysis, Funding acquisition, Investigation, Methodology, Writing – original draft

  Affiliation Faculty of Advanced Science and Technology, Department of Genetics, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

  https://orcid.org/0000-0002-7798-3610

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• Nayereh Ebrahimzadeh,

  Roles Methodology

  Affiliation Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Microbiology Research Center, Tehran, Iran

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• Hossein Fahimi ,

  Roles Conceptualization, Supervision, Writing – review & editing

  * E-mail: h.fahimi@iaups.ac.ir (HF); arfa.moshiri@nih.gov (AM)

  Affiliation Faculty of Advanced Science and Technology, Department of Genetics, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

  https://orcid.org/0000-0002-1744-8675

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• Arfa Moshiri

  Roles Project administration, Supervision

  * E-mail: h.fahimi@iaups.ac.ir (HF); arfa.moshiri@nih.gov (AM)

  Current address: Thoracic and GI Malignancies Branch, Center for Cancer Research, NCI, NIH, Bethesda, Maryland, United States of America

  Affiliation Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Microbiology Research Center, Tehran, Iran

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