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Open Access
Peer-reviewed
Research Article
A novel viral RNA detection method based on the combined use of trans-acting ribozymes and HCR-FRET analyses
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Leonardo Ferreira da Silva ,
Contributed equally to this work with: Leonardo Ferreira da Silva, Aisel Valle Garay
Roles Data curation, Formal analysis, Investigation, Methodology, Visualization, Writing – original draft, Writing – review & editing
Affiliation Laboratory of Synthetic Biology, Department of Genetics and Morphology, Institute of Biological Science, University of Brasília (UnB), Brasília, Federal District, Brazil
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Aisel Valle Garay ,
Contributed equally to this work with: Leonardo Ferreira da Silva, Aisel Valle Garay
Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Software, Visualization, Writing – original draft, Writing – review & editing
* E-mail: cintiacoelhom@unb.br (CMC); aiselvalle@gmail.com (AVG)
Affiliation Laboratory of Molecular Biophysics, Department of Cell Biology, Institute of Biological Sciences, University of Brasília (UnB), Brasília, Federal District, Brazil
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Pedro Felipe Queiroz,
Roles Data curation, Formal analysis, Investigation, Visualization, Writing – original draft, Writing – review & editing
Affiliation Laboratory of Synthetic Biology, Department of Genetics and Morphology, Institute of Biological Science, University of Brasília (UnB), Brasília, Federal District, Brazil
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Sophia Garcia de Resende,
Roles Data curation, Formal analysis, Investigation, Writing – original draft, Writing – review & editing
Affiliation Laboratory of Synthetic Biology, Department of Genetics and Morphology, Institute of Biological Science, University of Brasília (UnB), Brasília, Federal District, Brazil
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Mayna Gomide,
Roles Data curation, Formal analysis, Investigation, Methodology, Software, Writing – original draft, Writing – review & editing
Affiliation Laboratory of Synthetic Biology, Department of Genetics and Morphology, Institute of Biological Science, University of Brasília (UnB), Brasília, Federal District, Brazil
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Izadora Cristina Moreira de Oliveira,
Roles Data curation, Formal analysis, Investigation, Methodology, Writing – original draft, Writing – review & editing
Affiliation Laboratory of Molecular Biophysics, Department of Cell Biology, Institute of Biological Sciences, University of Brasília (UnB), Brasília, Federal District, Brazil
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Amanda Souza Bernasol,
Roles Data curation, Formal analysis, Investigation, Visualization, Writing – original draft, Writing – review & editing
Affiliation Laboratory of Molecular Biophysics, Department of Cell Biology, Institute of Biological Sciences, University of Brasília (UnB), Brasília, Federal District, Brazil
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Anibal Arce,
Roles Data curation, Formal analysis, Investigation, Software, Visualization, Writing – original draft, Writing – review & editing
Affiliation Institute for biological and medical engineering, Pontificia Universidad Católica de Chile, Santiago de Chile, Chile
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Liem Canet Santos,
Roles Data curation, Formal analysis, Investigation, Writing – original draft
Affiliation Laboratory of Molecular Biophysics, Department of Cell Biology, Institute of Biological Sciences, University of Brasília (UnB), Brasília, Federal District, Brazil
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Fernando Torres,
Roles Funding acquisition, Resources, Supervision, Writing – original draft, Writing – review & editing
Affiliation Laboratory of Molecular Biology, Department of Cell Biology, Institute of Biological Sciences, University of Brasília (UnB), Brasília, Federal District, Brazil
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Ildinete Silva-Pereira,
Roles Resources, Supervision, Writing – original draft, Writing – review & editing
Affiliation Laboratory of Molecular Biology of Pathogenic Fungi, Department of Cell Biology, Institute of Biological Sciences, University of Brasília (UnB), Brasília, Federal District, Brazil
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Sonia Maria de Freitas,
Roles Conceptualization, Formal analysis, Funding acquisition, Methodology, Project administration, Resources, Supervision, Writing – original draft, Writing – review & editing
Affiliation Laboratory of Molecular Biophysics, Department of Cell Biology, Institute of Biological Sciences, University of Brasília (UnB), Brasília, Federal District, Brazil
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Cíntia Marques Coelho
Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Supervision, Writing – original draft, Writing – review & editing
* E-mail: cintiacoelhom@unb.br (CMC); aiselvalle@gmail.com (AVG)
Affiliation Laboratory of Synthetic Biology, Department of Genetics and Morphology, Institute of Biological Science, University of Brasília (UnB), Brasília, Federal District, Brazil
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A novel viral RNA detection method based on the combined use of trans-acting ribozymes and HCR-FRET analyses
- Leonardo Ferreira da Silva,
- Aisel Valle Garay,
- Pedro Felipe Queiroz,
- Sophia Garcia de Resende,
- Mayna Gomide,
- Izadora Cristina Moreira de Oliveira,
- Amanda Souza Bernasol,
- Anibal Arce,
- Liem Canet Santos,
- Fernando Torres
- Published: September 26, 2024
- https://doi.org/10.1371/journal.pone.0310171