Rapid and efficient generation of mature retinal organoids derived from human pluripotent stem cells via optimized pharmacological modulation of Sonic hedgehog, activin A, and retinoic acid signal transduction

• Tokiyoshi Matsushita ,

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Validation, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: tokiyoshi.matsushita@santen.com

  Affiliations Faculty of Pharmaceutical Sciences, Department of Gene Regulation, Tokyo University of Science, Noda, Chiba, Japan, Product Discovery, Ophthalmology Innovation Center, Santen Pharmaceutical Co., Ltd., Ikoma, Nara, Japan

  https://orcid.org/0009-0000-7760-6885

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• Akishi Onishi,

  Roles Conceptualization, Project administration, Validation, Writing – review & editing

  Affiliations Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics Research, Kobe, Hyogo, Japan, Cell and Gene Therapy in Ophthalmology Laboratory, Baton Zone Program, RIKEN, Wako, Saitama, Japan

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• Takahiro Matsuyama,

  Roles Data curation, Formal analysis, Investigation, Methodology, Validation

  Affiliation Product Discovery, Ophthalmology Innovation Center, Santen Pharmaceutical Co., Ltd., Ikoma, Nara, Japan

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• Tomohiro Masuda,

  Roles Data curation, Formal analysis, Investigation, Methodology

  Affiliations Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics Research, Kobe, Hyogo, Japan, Cell and Gene Therapy in Ophthalmology Laboratory, Baton Zone Program, RIKEN, Wako, Saitama, Japan

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• Yoko Ogino,

  Roles Project administration

  Affiliation Faculty of Pharmaceutical Sciences, Department of Gene Regulation, Tokyo University of Science, Noda, Chiba, Japan

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• Masaaki Kageyama,

  Roles Supervision, Writing – review & editing

  Affiliation Product Discovery, Ophthalmology Innovation Center, Santen Pharmaceutical Co., Ltd., Ikoma, Nara, Japan

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• Masayo Takahashi,

  Roles Supervision

  Affiliations Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics Research, Kobe, Hyogo, Japan, Cell and Gene Therapy in Ophthalmology Laboratory, Baton Zone Program, RIKEN, Wako, Saitama, Japan

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• Fumiaki Uchiumi

  Roles Supervision, Writing – review & editing

  Affiliation Faculty of Pharmaceutical Sciences, Department of Gene Regulation, Tokyo University of Science, Noda, Chiba, Japan

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