Optimization of culture condition for Spodoptera frugiperda by design of experiment approach and evaluation of its effect on the expression of hemagglutinin protein of influenza virus

Fatemeh Alizadeh; Hamideh Aghajani; Fereidoun Mahboudi; Yeganeh Talebkhan; Ehsan Arefian; Sepideh Samavat; Rouhollah Raufi

doi:10.1371/journal.pone.0308547

Loading metrics

Open Access

Peer-reviewed

Research Article

Optimization of culture condition for Spodoptera frugiperda by design of experiment approach and evaluation of its effect on the expression of hemagglutinin protein of influenza virus

Fatemeh Alizadeh,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Validation, Visualization, Writing – original draft, Writing – review & editing

Affiliations Biotechnology Research Center, Department of Medical Biotechnology, Pasteur Institute of Iran, Tehran, Iran, Department of Research & Development, AryoGen Pharmed Inc., Karaj, Iran
⨯
Hamideh Aghajani ,

Roles Conceptualization, Methodology, Project administration

* E-mail: talebkhan@pasteur.ac.ir, ytgaroosi@gmail.com (YT); aghajanih@aryogen.com (HA)

Affiliation Department of Research & Development, AryoGen Pharmed Inc., Karaj, Iran
⨯
Fereidoun Mahboudi,

Roles Conceptualization, Funding acquisition, Project administration

Affiliations Biotechnology Research Center, Department of Medical Biotechnology, Pasteur Institute of Iran, Tehran, Iran, Department of Research & Development, AryoGen Pharmed Inc., Karaj, Iran
⨯
Yeganeh Talebkhan ,

Roles Data curation, Formal analysis, Methodology, Project administration, Validation, Visualization, Writing – review & editing

* E-mail: talebkhan@pasteur.ac.ir, ytgaroosi@gmail.com (YT); aghajanih@aryogen.com (HA)

Affiliation Biotechnology Research Center, Department of Medical Biotechnology, Pasteur Institute of Iran, Tehran, Iran

https://orcid.org/0000-0003-1988-4419

⨯
Ehsan Arefian,

Roles Methodology, Project administration, Writing – review & editing

Affiliation Molecular Virology Lab, Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran
⨯
Sepideh Samavat,

Roles Methodology

Affiliation Department of Research & Development, AryoGen Pharmed Inc., Karaj, Iran
⨯
Rouhollah Raufi

Roles Investigation

Affiliation Department of Research & Development, AryoGen Pharmed Inc., Karaj, Iran
⨯

Optimization of culture condition for Spodoptera frugiperda by design of experiment approach and evaluation of its effect on the expression of hemagglutinin protein of influenza virus

Fatemeh Alizadeh,
Hamideh Aghajani,
Fereidoun Mahboudi,
Yeganeh Talebkhan,
Ehsan Arefian,
Sepideh Samavat,
Rouhollah Raufi

Published: August 16, 2024
https://doi.org/10.1371/journal.pone.0308547

Reader Comments (0)

Post a new comment on this article

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

Protein expression
Is the Subject Area "Protein expression" applicable to this article?

Thanks for your feedback.
Experimental design
Is the Subject Area "Experimental design" applicable to this article?

Thanks for your feedback.
Cholesterol
Is the Subject Area "Cholesterol" applicable to this article?

Thanks for your feedback.
Recombinant proteins
Is the Subject Area "Recombinant proteins" applicable to this article?

Thanks for your feedback.
Viable cell counting
Is the Subject Area "Viable cell counting" applicable to this article?

Thanks for your feedback.
Cell cultures
Is the Subject Area "Cell cultures" applicable to this article?

Thanks for your feedback.
Insects
Is the Subject Area "Insects" applicable to this article?

Thanks for your feedback.
Viral vaccines
Is the Subject Area "Viral vaccines" applicable to this article?

Thanks for your feedback.