A reliable and quick method for screening alternative splicing variants for low-abundance genes

Yanchun Zhang; Wubin Qu; Ruifen Yan; Huqi Liu; Chenggang Zhang; Zhihui Li; Guofu Dong

doi:10.1371/journal.pone.0305201

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Open Access

Peer-reviewed

Research Article

A reliable and quick method for screening alternative splicing variants for low-abundance genes

Yanchun Zhang,

Roles Writing – original draft

Affiliations Department of Blood Transfusion Medicine, The Seventh Medical Center of PLA General Hospital, Beijing, China, Laboratory of Electromagnetic Biological Effects, Beijing Institute of Radiation and Medicine, Beijing, China
⨯
Wubin Qu,

Roles Software

Affiliation Laboratory of Electromagnetic Biological Effects, Beijing Institute of Radiation and Medicine, Beijing, China
⨯
Ruifen Yan,

Roles Validation

Affiliations Laboratory of Electromagnetic Biological Effects, Beijing Institute of Radiation and Medicine, Beijing, China, College of Life Science, Northwest Agriculture and Forest University, Yangling, China
⨯
Huqi Liu,

Roles Formal analysis

Affiliation College of Life Science, Northwest Agriculture and Forest University, Yangling, China
⨯
Chenggang Zhang,

Roles Writing – review & editing

Affiliation Laboratory of Electromagnetic Biological Effects, Beijing Institute of Radiation and Medicine, Beijing, China

https://orcid.org/0000-0002-4521-3304

⨯
Zhihui Li ,

Roles Writing – review & editing

* E-mail: l3521025015@163.com (ZL); dongguofu052@126.com (GD)

Affiliation Laboratory of Electromagnetic Biological Effects, Beijing Institute of Radiation and Medicine, Beijing, China

https://orcid.org/0000-0002-7042-5966

⨯
Guofu Dong

Roles Writing – review & editing

* E-mail: l3521025015@163.com (ZL); dongguofu052@126.com (GD)

Affiliation Laboratory of Electromagnetic Biological Effects, Beijing Institute of Radiation and Medicine, Beijing, China
⨯

A reliable and quick method for screening alternative splicing variants for low-abundance genes

Yanchun Zhang,
Wubin Qu,
Ruifen Yan,
Huqi Liu,
Chenggang Zhang,
Zhihui Li,
Guofu Dong

Published: June 27, 2024
https://doi.org/10.1371/journal.pone.0305201

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Genetic screens
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Polymerase chain reaction
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Caenorhabditis elegans
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Microarrays
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Gene expression
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Alternative splicing
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Reverse transcription
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Sequence databases
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