Preanalytical considerations for clinical assays of circulating human miRNA-451a, miRNA-423-5p and miRNA-199a-3p for diagnostic purposes

Dinesh S. Chandel; Wesley A. Tom; Chao Jiang; Gary Krzyzanowski; Nirmalee Fernando; Appolinaire Olou; M. Rohan Fernando

doi:10.1371/journal.pone.0303598

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Open Access

Peer-reviewed

Research Article

Preanalytical considerations for clinical assays of circulating human miRNA-451a, miRNA-423-5p and miRNA-199a-3p for diagnostic purposes

Dinesh S. Chandel,

Roles Data curation, Formal analysis, Investigation, Methodology, Writing – review & editing

Affiliation Molecular Diagnostic Research Laboratory, Center for Sensory Neuroscience, Boys Town National Research Hospital, Omaha, NE, United States of America
⨯
Wesley A. Tom,

Roles Formal analysis, Methodology, Software, Validation, Visualization

Affiliation Molecular Diagnostic Research Laboratory, Center for Sensory Neuroscience, Boys Town National Research Hospital, Omaha, NE, United States of America
⨯
Chao Jiang,

Roles Formal analysis, Investigation, Methodology, Writing – review & editing

Affiliation Molecular Diagnostic Research Laboratory, Center for Sensory Neuroscience, Boys Town National Research Hospital, Omaha, NE, United States of America
⨯
Gary Krzyzanowski,

Roles Formal analysis, Investigation, Methodology, Writing – review & editing

Affiliation Molecular Diagnostic Research Laboratory, Center for Sensory Neuroscience, Boys Town National Research Hospital, Omaha, NE, United States of America
⨯
Nirmalee Fernando,

Roles Investigation, Methodology, Writing – review & editing

Affiliation Molecular Diagnostic Research Laboratory, Center for Sensory Neuroscience, Boys Town National Research Hospital, Omaha, NE, United States of America
⨯
Appolinaire Olou,

Roles Formal analysis, Methodology, Visualization, Writing – review & editing

Affiliation Molecular Diagnostic Research Laboratory, Center for Sensory Neuroscience, Boys Town National Research Hospital, Omaha, NE, United States of America
⨯
M. Rohan Fernando

Roles Conceptualization, Funding acquisition, Supervision, Writing – original draft

* E-mail: M.Rohan.Fernando@boystown.org

Affiliation Molecular Diagnostic Research Laboratory, Center for Sensory Neuroscience, Boys Town National Research Hospital, Omaha, NE, United States of America

https://orcid.org/0000-0002-3596-3160

⨯

Preanalytical considerations for clinical assays of circulating human miRNA-451a, miRNA-423-5p and miRNA-199a-3p for diagnostic purposes

Dinesh S. Chandel,
Wesley A. Tom,
Chao Jiang,
Gary Krzyzanowski,
Nirmalee Fernando,
Appolinaire Olou,
M. Rohan Fernando

Published: May 20, 2024
https://doi.org/10.1371/journal.pone.0303598

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