qPCR assay for detection of Woodchuck Hepatitis Virus Post-Transcriptional Regulatory Elements from CAR-T and TCR-T cells in fresh and formalin-fixed tissue

Shalini Pullarkat; Graeme Black; Marie Bleakley; Denise Buenrostro; Aude G. Chapuis; Alexandre V. Hirayama; Carla A. Jaeger-Ruckstuhl; Erik L. Kimble; Bo M. Lee; David G. Maloney; Jerald Radich; Brandon W. Seaton; Jennifer M. Specht; Cameron J. Turtle; David W. Woolston; Jocelyn H. Wright; Cecilia C. S. Yeung

doi:10.1371/journal.pone.0303057

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Open Access

Peer-reviewed

Research Article

qPCR assay for detection of Woodchuck Hepatitis Virus Post-Transcriptional Regulatory Elements from CAR-T and TCR-T cells in fresh and formalin-fixed tissue

Shalini Pullarkat,

Roles Conceptualization, Formal analysis, Investigation, Methodology, Project administration, Resources, Validation, Visualization, Writing – original draft, Writing – review & editing

Affiliation Translational Science and Therapeutics Division, Fred Hutchinson Cancer Center, Seattle, Washington, United States of America

https://orcid.org/0009-0000-9024-5842

⨯
Graeme Black,

Roles Resources

Affiliation Translational Science and Therapeutics Division, Fred Hutchinson Cancer Center, Seattle, Washington, United States of America
⨯
Marie Bleakley,

Roles Resources, Writing – review & editing

Affiliations Translational Science and Therapeutics Division, Fred Hutchinson Cancer Center, Seattle, Washington, United States of America, Clinical Research Division, Fred Hutchinson Cancer Center, Seattle, Washington, United States of America, Department of Pediatrics, University of Washington, Seattle, Washington, United States of America, Program in Immunology, University of Washington, Seattle, Washington, United States of America
⨯
Denise Buenrostro,

Roles Resources

Affiliations Clinical Research Division, Fred Hutchinson Cancer Center, Seattle, Washington, United States of America, Program in Immunology, University of Washington, Seattle, Washington, United States of America
⨯
Aude G. Chapuis,

Roles Resources

Affiliations Translational Science and Therapeutics Division, Fred Hutchinson Cancer Center, Seattle, Washington, United States of America, Clinical Research Division, Fred Hutchinson Cancer Center, Seattle, Washington, United States of America, Program in Immunology, University of Washington, Seattle, Washington, United States of America, Department of Medicine, Division of Hematology and Oncology, University of Washington, Seattle, Washington, United States of America
⨯
Alexandre V. Hirayama,

Roles Conceptualization, Resources

Affiliations Clinical Research Division, Fred Hutchinson Cancer Center, Seattle, Washington, United States of America, Department of Medicine, Division of Hematology and Oncology, University of Washington, Seattle, Washington, United States of America
⨯
Carla A. Jaeger-Ruckstuhl,

Roles Resources, Writing – review & editing

Affiliation Translational Science and Therapeutics Division, Fred Hutchinson Cancer Center, Seattle, Washington, United States of America
⨯
Erik L. Kimble,

Roles Conceptualization, Methodology, Resources, Writing – review & editing

Affiliations Translational Science and Therapeutics Division, Fred Hutchinson Cancer Center, Seattle, Washington, United States of America, Department of Medicine, Division of Hematology and Oncology, University of Washington, Seattle, Washington, United States of America

https://orcid.org/0000-0002-1885-1773

⨯
Bo M. Lee,

Roles Resources

Affiliation Translational Science and Therapeutics Division, Fred Hutchinson Cancer Center, Seattle, Washington, United States of America
⨯
David G. Maloney,

Roles Resources

Affiliations Translational Science and Therapeutics Division, Fred Hutchinson Cancer Center, Seattle, Washington, United States of America, Clinical Research Division, Fred Hutchinson Cancer Center, Seattle, Washington, United States of America, Department of Medicine, Division of Hematology and Oncology, University of Washington, Seattle, Washington, United States of America
⨯
Jerald Radich,

Roles Resources, Supervision, Writing – review & editing

Affiliations Translational Science and Therapeutics Division, Fred Hutchinson Cancer Center, Seattle, Washington, United States of America, Clinical Research Division, Fred Hutchinson Cancer Center, Seattle, Washington, United States of America
⨯
Brandon W. Seaton,

Roles Resources

Affiliation Translational Science and Therapeutics Division, Fred Hutchinson Cancer Center, Seattle, Washington, United States of America
⨯
Jennifer M. Specht,

Roles Funding acquisition, Resources, Writing – review & editing

Affiliations Clinical Research Division, Fred Hutchinson Cancer Center, Seattle, Washington, United States of America, Department of Medicine, Division of Hematology and Oncology, University of Washington, Seattle, Washington, United States of America
⨯
Cameron J. Turtle,

Roles Conceptualization, Methodology

Affiliations Translational Science and Therapeutics Division, Fred Hutchinson Cancer Center, Seattle, Washington, United States of America, Clinical Research Division, Fred Hutchinson Cancer Center, Seattle, Washington, United States of America, Department of Medicine, Division of Hematology and Oncology, University of Washington, Seattle, Washington, United States of America, Integrated Immunotherapy Research Center, Fred Hutchinson Cancer Center, Seattle, WA, United States of America
⨯
David W. Woolston,

Roles Conceptualization, Methodology, Visualization, Writing – review & editing

Affiliation Translational Science and Therapeutics Division, Fred Hutchinson Cancer Center, Seattle, Washington, United States of America
⨯
Jocelyn H. Wright,

Roles Conceptualization, Methodology, Project administration, Resources, Supervision, Writing – original draft, Writing – review & editing

Affiliations Translational Science and Therapeutics Division, Fred Hutchinson Cancer Center, Seattle, Washington, United States of America, Program in Immunology, University of Washington, Seattle, Washington, United States of America
⨯
[ ... ],
Cecilia C. S. Yeung

Roles Conceptualization, Funding acquisition, Methodology, Project administration, Resources, Supervision, Writing – review & editing

* E-mail: cyeung5@uw.edu

Affiliations Translational Science and Therapeutics Division, Fred Hutchinson Cancer Center, Seattle, Washington, United States of America, Clinical Research Division, Fred Hutchinson Cancer Center, Seattle, Washington, United States of America, Department of Laboratory Medicine and Pathology, University of Washington School of Medicine, Seattle, Washington, United States of America

https://orcid.org/0000-0001-6799-2022

⨯
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qPCR assay for detection of Woodchuck Hepatitis Virus Post-Transcriptional Regulatory Elements from CAR-T and TCR-T cells in fresh and formalin-fixed tissue

Shalini Pullarkat,
Graeme Black,
Marie Bleakley,
Denise Buenrostro,
Aude G. Chapuis,
Alexandre V. Hirayama,
Carla A. Jaeger-Ruckstuhl,
Erik L. Kimble,
Bo M. Lee,
David G. Maloney

Published: June 6, 2024
https://doi.org/10.1371/journal.pone.0303057

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