Validation of a real-time quaking-induced conversion (RT-QuIC) assay protocol to detect chronic wasting disease using rectal mucosa of naturally infected, pre-clinical white-tailed deer (Odocoileus virginianus)

• Robert B. Piel III,

  Roles Data curation, Investigation, Methodology, Visualization, Writing – original draft, Writing – review & editing

  Affiliations U.S. Department of Agriculture, Agricultural Research Service, Animal Disease Research Unit, Pullman, Washington, United States of America, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, United States of America

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• Susan E. Veneziano,

  Roles Investigation, Methodology, Writing – review & editing

  Affiliation U.S. Department of Agriculture, Agricultural Research Service, National Animal Disease Center, Virus and Prion Research Unit, Ames, Iowa, United States of America

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• Eric M. Nicholson,

  Roles Funding acquisition, Methodology, Project administration, Supervision, Writing – review & editing

  Affiliation U.S. Department of Agriculture, Agricultural Research Service, National Animal Disease Center, Virus and Prion Research Unit, Ames, Iowa, United States of America

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• Daniel P. Walsh,

  Roles Conceptualization, Funding acquisition, Methodology, Project administration, Resources, Supervision, Writing – review & editing

  Affiliations U.S. Geological Survey, Montana Cooperative Wildlife Research Unit, Missoula, Montana, United States of America, Wildlife Biology Program, University of Montana, Missoula, Montana, United States of America

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• Aaron D. Lomax,

  Roles Investigation, Methodology, Resources, Writing – review & editing

  Current address: Varizymes, Middleton, Wisconsin, United States of America

  Affiliation Department of Soil Science, University of Wisconsin, Madison, Wisconsin, United States of America

  https://orcid.org/0000-0002-1805-3579

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• Tracy A. Nichols,

  Roles Conceptualization, Data curation, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Writing – review & editing

  Affiliation U.S. Department of Agriculture, Animal Plant Health Inspection Service, Veterinary Services, Fort Collins, Colorado, United States of America

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• Christopher M. Seabury,

  Roles Investigation, Writing – review & editing

  Affiliation Department of Veterinary Pathobiology, Texas A&M University, College Station, Texas, United States of America

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• David A. Schneider

  Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Project administration, Resources, Software, Supervision, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: david.schneider1@usda.gov

  Affiliations U.S. Department of Agriculture, Agricultural Research Service, Animal Disease Research Unit, Pullman, Washington, United States of America, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, United States of America

  https://orcid.org/0000-0001-9659-6731

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