Formamide denaturation of double-stranded DNA for fluorescence in situ hybridization (FISH) distorts nanoscale chromatin structure

• Anne R. Shim ,

  Contributed equally to this work with: Anne R. Shim, Jane Frederick, Emily M. Pujadas

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Software, Visualization, Writing – original draft, Writing – review & editing

  Affiliations Department of Biomedical Engineering, Northwestern University, Evanston, Illinois, United States of America, Center for Physical Genomics and Engineering, Northwestern University, Evanston, Illinois, United States of America, Chemistry of Life Processes Institute, Northwestern University, Evanston, Illinois, United States of America

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• Jane Frederick ,

  Contributed equally to this work with: Anne R. Shim, Jane Frederick, Emily M. Pujadas

  Roles Data curation, Formal analysis, Investigation, Methodology, Software, Validation, Visualization, Writing – original draft, Writing – review & editing

  Affiliations Department of Biomedical Engineering, Northwestern University, Evanston, Illinois, United States of America, Center for Physical Genomics and Engineering, Northwestern University, Evanston, Illinois, United States of America, Chemistry of Life Processes Institute, Northwestern University, Evanston, Illinois, United States of America

  https://orcid.org/0000-0003-3007-9197

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• Emily M. Pujadas ,

  Contributed equally to this work with: Anne R. Shim, Jane Frederick, Emily M. Pujadas

  Roles Investigation, Validation, Writing – original draft, Writing – review & editing

  Affiliations Department of Biomedical Engineering, Northwestern University, Evanston, Illinois, United States of America, Center for Physical Genomics and Engineering, Northwestern University, Evanston, Illinois, United States of America, Chemistry of Life Processes Institute, Northwestern University, Evanston, Illinois, United States of America

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• Tiffany Kuo,

  Roles Investigation, Writing – review & editing

  Affiliations Department of Biomedical Engineering, Northwestern University, Evanston, Illinois, United States of America, Center for Physical Genomics and Engineering, Northwestern University, Evanston, Illinois, United States of America, Chemistry of Life Processes Institute, Northwestern University, Evanston, Illinois, United States of America

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• I. Chae Ye,

  Roles Investigation, Validation, Writing – review & editing

  Affiliations Department of Biomedical Engineering, Northwestern University, Evanston, Illinois, United States of America, Center for Physical Genomics and Engineering, Northwestern University, Evanston, Illinois, United States of America, Chemistry of Life Processes Institute, Northwestern University, Evanston, Illinois, United States of America

  https://orcid.org/0000-0002-5072-8900

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• Joshua A. Pritchard,

  Roles Investigation

  Affiliations Department of Biomedical Engineering, Northwestern University, Evanston, Illinois, United States of America, Center for Physical Genomics and Engineering, Northwestern University, Evanston, Illinois, United States of America, Chemistry of Life Processes Institute, Northwestern University, Evanston, Illinois, United States of America

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• Cody L. Dunton,

  Roles Investigation, Writing – review & editing

  Affiliations Department of Biomedical Engineering, Northwestern University, Evanston, Illinois, United States of America, Center for Physical Genomics and Engineering, Northwestern University, Evanston, Illinois, United States of America, Chemistry of Life Processes Institute, Northwestern University, Evanston, Illinois, United States of America

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• Paola Carrillo Gonzalez,

  Roles Investigation

  Affiliations Department of Biomedical Engineering, Northwestern University, Evanston, Illinois, United States of America, Center for Physical Genomics and Engineering, Northwestern University, Evanston, Illinois, United States of America, Chemistry of Life Processes Institute, Northwestern University, Evanston, Illinois, United States of America

  https://orcid.org/0000-0003-2988-0913

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• Nicolas Acosta,

  Roles Software

  Affiliations Department of Biomedical Engineering, Northwestern University, Evanston, Illinois, United States of America, Center for Physical Genomics and Engineering, Northwestern University, Evanston, Illinois, United States of America, Chemistry of Life Processes Institute, Northwestern University, Evanston, Illinois, United States of America

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• Surbhi Jain,

  Roles Investigation, Methodology, Writing – original draft, Writing – review & editing

  Affiliations Department of Biomedical Engineering, Northwestern University, Evanston, Illinois, United States of America, Center for Physical Genomics and Engineering, Northwestern University, Evanston, Illinois, United States of America, Chemistry of Life Processes Institute, Northwestern University, Evanston, Illinois, United States of America

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• Nicholas M. Anthony,

  Roles Software

  Affiliations Department of Biomedical Engineering, Northwestern University, Evanston, Illinois, United States of America, Center for Physical Genomics and Engineering, Northwestern University, Evanston, Illinois, United States of America, Chemistry of Life Processes Institute, Northwestern University, Evanston, Illinois, United States of America

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• Luay M. Almassalha,

  Roles Writing – review & editing

  Affiliations Department of Biomedical Engineering, Northwestern University, Evanston, Illinois, United States of America, Center for Physical Genomics and Engineering, Northwestern University, Evanston, Illinois, United States of America, Chemistry of Life Processes Institute, Northwestern University, Evanston, Illinois, United States of America, Department of Gastroenterology and Hepatology, Northwestern Memorial Hospital, Chicago, Illinois, United States of America

  https://orcid.org/0000-0001-9355-7681

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• Igal Szleifer,

  Roles Conceptualization, Funding acquisition, Project administration, Resources, Supervision, Writing – original draft, Writing – review & editing

  Affiliations Department of Biomedical Engineering, Northwestern University, Evanston, Illinois, United States of America, Center for Physical Genomics and Engineering, Northwestern University, Evanston, Illinois, United States of America, Department of Chemistry, Northwestern University, Evanston, Illinois, United States of America

  https://orcid.org/0000-0002-8708-0335

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• Vadim Backman

  Roles Conceptualization, Funding acquisition, Project administration, Resources, Supervision, Writing – original draft, Writing – review & editing

  * E-mail: v-backman@northwestern.edu

  Affiliations Department of Biomedical Engineering, Northwestern University, Evanston, Illinois, United States of America, Center for Physical Genomics and Engineering, Northwestern University, Evanston, Illinois, United States of America, Chemistry of Life Processes Institute, Northwestern University, Evanston, Illinois, United States of America

  https://orcid.org/0000-0003-1981-1818

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