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Open Access
Peer-reviewed
Research Article
A novel system to culture human intestinal organoids under physiological oxygen content to study microbial-host interaction
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Tatiana Y. Fofanova ,
Contributed equally to this work with: Tatiana Y. Fofanova, Umesh C. Karandikar
Roles Conceptualization, Data curation, Investigation, Project administration, Software, Writing – original draft
Affiliation Alkek Centre for Metagenomics and Microbiome Research, Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, United States of America
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Umesh C. Karandikar ,
Contributed equally to this work with: Tatiana Y. Fofanova, Umesh C. Karandikar
Roles Writing – original draft, Writing – review & editing
Affiliation Alkek Centre for Metagenomics and Microbiome Research, Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, United States of America
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Jennifer M. Auchtung,
Roles Investigation, Methodology, Writing – original draft, Writing – review & editing
Affiliations Alkek Centre for Metagenomics and Microbiome Research, Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, United States of America, Department of Food Science and Technology, University of Nebraska-Lincoln, Lincoln, NE, United States of America
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Reid L. Wilson,
Roles Investigation, Methodology
Affiliations Department of Bioengineering, Rice University, Houston, TX, United States of America, Medical Scientist Training Program, Baylor College of Medicine, Houston, TX, United States of America
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Antonio J. Valentin,
Roles Investigation
Affiliation Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, United States of America
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Robert A. Britton,
Roles Resources
Affiliation Alkek Centre for Metagenomics and Microbiome Research, Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, United States of America
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K. Jane Grande-Allen,
Roles Resources
Affiliation Department of Bioengineering, Rice University, Houston, TX, United States of America
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Mary K. Estes,
Roles Funding acquisition, Resources, Writing – review & editing
Affiliations Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, United States of America, Department of Medicine, Baylor College of Medicine, Houston, TX, United States of America
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Kristi Hoffman,
Roles Project administration
Affiliation Alkek Centre for Metagenomics and Microbiome Research, Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, United States of America
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Sashirekha Ramani,
Roles Writing – review & editing
Affiliation Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, United States of America
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Christopher J. Stewart ,
Roles Investigation, Writing – original draft, Writing – review & editing
* E-mail: christopher.stewart@newcastle.ac.uk (CJS); jpetrosi@bcm.edu (JFP)
Affiliations Alkek Centre for Metagenomics and Microbiome Research, Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, United States of America, Translational and Clinical Research Institute, Newcastle University, Newcastle, United Kingdom
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Joseph F. Petrosino
Roles Conceptualization, Resources
* E-mail: christopher.stewart@newcastle.ac.uk (CJS); jpetrosi@bcm.edu (JFP)
Affiliations Alkek Centre for Metagenomics and Microbiome Research, Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, United States of America, Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, United States of America
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A novel system to culture human intestinal organoids under physiological oxygen content to study microbial-host interaction
- Tatiana Y. Fofanova,
- Umesh C. Karandikar,
- Jennifer M. Auchtung,
- Reid L. Wilson,
- Antonio J. Valentin,
- Robert A. Britton,
- K. Jane Grande-Allen,
- Mary K. Estes,
- Kristi Hoffman,
- Sashirekha Ramani
- Published: July 25, 2024
- https://doi.org/10.1371/journal.pone.0300666