High-volume, label-free imaging for quantifying single-cell dynamics in induced pluripotent stem cell colonies

Anthony J. Asmar; Zackery A. Benson; Adele P. Peskin; Joe Chalfoun; Mylene Simon; Michael Halter; Anne L. Plant

doi:10.1371/journal.pone.0298446

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Open Access

Peer-reviewed

Research Article

High-volume, label-free imaging for quantifying single-cell dynamics in induced pluripotent stem cell colonies

Anthony J. Asmar ,

Contributed equally to this work with: Anthony J. Asmar, Zackery A. Benson

Roles Data curation, Formal analysis, Investigation, Methodology, Software, Visualization, Writing – original draft, Writing – review & editing

* E-mail: anthony.asmar@nist.gov

Affiliation Biosystems and Biomaterials Division Material Measurement Lab, NIST Gaithersburg, Gaithersburg, Maryland, United States of America

https://orcid.org/0000-0002-4843-0785

⨯
Zackery A. Benson ,

Contributed equally to this work with: Anthony J. Asmar, Zackery A. Benson

Roles Formal analysis, Investigation, Methodology, Software, Visualization, Writing – original draft, Writing – review & editing

Affiliation Biosystems and Biomaterials Division Material Measurement Lab, NIST Gaithersburg, Gaithersburg, Maryland, United States of America
⨯
Adele P. Peskin,

Roles Methodology, Software, Writing – review & editing

Affiliation Software and Systems Division Information Technology Lab, NIST Gaithersburg, Gaithersburg, Maryland, United States of America
⨯
Joe Chalfoun,

Roles Writing – review & editing

Affiliation Software and Systems Division Information Technology Lab, NIST Gaithersburg, Gaithersburg, Maryland, United States of America
⨯
Mylene Simon,

Roles Visualization

Affiliation Software and Systems Division Information Technology Lab, NIST Gaithersburg, Gaithersburg, Maryland, United States of America
⨯
Michael Halter,

Roles Conceptualization, Methodology, Project administration, Supervision, Writing – original draft, Writing – review & editing

Affiliation Biosystems and Biomaterials Division Material Measurement Lab, NIST Gaithersburg, Gaithersburg, Maryland, United States of America
⨯
Anne L. Plant

Roles Conceptualization, Project administration, Supervision, Writing – original draft, Writing – review & editing

Affiliation Biosystems and Biomaterials Division Material Measurement Lab, NIST Gaithersburg, Gaithersburg, Maryland, United States of America
⨯

High-volume, label-free imaging for quantifying single-cell dynamics in induced pluripotent stem cell colonies

Anthony J. Asmar,
Zackery A. Benson,
Adele P. Peskin,
Joe Chalfoun,
Mylene Simon,
Michael Halter,
Anne L. Plant

Published: February 20, 2024
https://doi.org/10.1371/journal.pone.0298446

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Mitosis
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Induced pluripotent stem cells
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Cell cycle and cell division
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Stem cell lines
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