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Open Access
Peer-reviewed
Research Article
A semi-automated cell tracking protocol for quantitative analyses of neutrophil swarming to sterile and S. aureus contaminated bone implants in a mouse femur model
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Sashank Lekkala,
Roles Data curation, Formal analysis, Methodology, Software, Validation, Visualization, Writing – original draft, Writing – review & editing
Affiliations Department of Biomedical Engineering, University of Rochester, Rochester, New York, United States of America, Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, New York, United States of America
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Youliang Ren,
Roles Data curation, Formal analysis, Methodology, Writing – review & editing
Affiliations Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, New York, United States of America, Department of Orthopaedics and Rehabilitation, University of Rochester Medical Center, Rochester, New York, United States of America
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Jason Weeks,
Roles Formal analysis, Methodology, Software, Writing – review & editing
Affiliation Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, New York, United States of America
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Kevin Lee,
Roles Data curation, Formal analysis, Methodology, Software, Validation, Writing – review & editing
Affiliations Department of Biomedical Engineering, University of Rochester, Rochester, New York, United States of America, Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, New York, United States of America
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Allie Jia Hui Tay,
Roles Formal analysis, Writing – review & editing
Affiliations Department of Biomedical Engineering, University of Rochester, Rochester, New York, United States of America, Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, New York, United States of America
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Bei Liu,
Roles Formal analysis, Writing – review & editing
Affiliation Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, New York, United States of America
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Thomas Xue,
Roles Formal analysis, Methodology, Writing – review & editing
Affiliations Department of Biomedical Engineering, University of Rochester, Rochester, New York, United States of America, Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, New York, United States of America
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Joshua Rainbolt,
Roles Methodology, Writing – review & editing
Affiliation Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, New York, United States of America
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Chao Xie,
Roles Conceptualization, Data curation, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Writing – review & editing
Affiliations Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, New York, United States of America, Department of Orthopaedics and Rehabilitation, University of Rochester Medical Center, Rochester, New York, United States of America
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Edward M. Schwarz ,
Roles Conceptualization, Data curation, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Writing – review & editing
* E-mail: ShuChi_Yeh@URMC.Rochester.edu (SCAY); Edward_Schwarz@URMC.rochester.edu (EMS)
Affiliations Department of Biomedical Engineering, University of Rochester, Rochester, New York, United States of America, Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, New York, United States of America, Department of Orthopaedics and Rehabilitation, University of Rochester Medical Center, Rochester, New York, United States of America
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Shu-Chi A. Yeh
Roles Conceptualization, Data curation, Funding acquisition, Investigation, Methodology, Project administration, Resources, Software, Supervision, Validation, Writing – review & editing
* E-mail: ShuChi_Yeh@URMC.Rochester.edu (SCAY); Edward_Schwarz@URMC.rochester.edu (EMS)
Affiliations Department of Biomedical Engineering, University of Rochester, Rochester, New York, United States of America, Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, New York, United States of America, Department of Orthopaedics and Rehabilitation, University of Rochester Medical Center, Rochester, New York, United States of America, Department of Physiology/Pharmacology, University of Rochester Medical Center, Rochester, New York, United States of America
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A semi-automated cell tracking protocol for quantitative analyses of neutrophil swarming to sterile and S. aureus contaminated bone implants in a mouse femur model
- Sashank Lekkala,
- Youliang Ren,
- Jason Weeks,
- Kevin Lee,
- Allie Jia Hui Tay,
- Bei Liu,
- Thomas Xue,
- Joshua Rainbolt,
- Chao Xie,
- Edward M. Schwarz
- Published: June 20, 2024
- https://doi.org/10.1371/journal.pone.0296140