Performance characteristics of a polymerase chain reaction-based assay for the detection of EGFR mutations in plasma cell-free DNA from patients with non-small cell lung cancer using cell-free DNA collection tubes

Theresa May; Michelle S. Clement; Harkanwal Halait; Alexander Kohlmann; Milena Kohlmann; Jason Lai; Nitta Lee; Xiaocheng Li-Sucholeiki; Peter Meldgaard; Snehal Joshi; Sidney Scudder; Neelima Shrestha; Boe Sorensen; Marilyn Kiral; Patrick O’Donnell

doi:10.1371/journal.pone.0295987

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Open Access

Peer-reviewed

Research Article

Performance characteristics of a polymerase chain reaction-based assay for the detection of EGFR mutations in plasma cell-free DNA from patients with non-small cell lung cancer using cell-free DNA collection tubes

Theresa May ,

Roles Conceptualization, Formal analysis, Methodology, Visualization, Writing – review & editing

* E-mail: theresa.may@roche.com

Affiliation Research and Development, Roche Molecular Systems, Inc., Pleasanton, CA, United States of America

https://orcid.org/0009-0004-2639-7517

⨯
Michelle S. Clement,

Roles Data curation, Investigation, Writing – review & editing

Affiliation Department of Clinical Biochemistry, Aarhus University Hospital, Aarhus, Denmark
⨯
Harkanwal Halait,

Roles Visualization, Writing – review & editing

Affiliation Research and Development, Roche Molecular Systems, Inc., Pleasanton, CA, United States of America
⨯
Alexander Kohlmann,

Roles Visualization, Writing – review & editing

Affiliation Oncology R&D, Precision Medicine and Biosamples, AstraZeneca, Gaithersburg, MD, United States of America
⨯
Milena Kohlmann,

Roles Visualization, Writing – review & editing

Affiliation Oncology R&D, Precision Medicine and Biosamples, AstraZeneca, Gaithersburg, MD, United States of America
⨯
Jason Lai,

Roles Visualization, Writing – review & editing

Current address: Genentech, Inc., South San Francisco, CA, United States of America

Affiliation Research and Development, Roche Molecular Systems, Inc., Pleasanton, CA, United States of America
⨯
Nitta Lee,

Roles Data curation, Formal analysis, Visualization, Writing – review & editing

Affiliation Research and Development, Roche Molecular Systems, Inc., Pleasanton, CA, United States of America
⨯
Xiaocheng Li-Sucholeiki,

Roles Visualization, Writing – review & editing

Affiliation Oncology R&D, Precision Medicine and Biosamples, AstraZeneca, Boston, MA, United States of America
⨯
Peter Meldgaard,

Roles Visualization, Writing – review & editing

Affiliation Department of Oncology, Aarhus University Hospital, Aarhus, Denmark

https://orcid.org/0000-0002-5788-4463

⨯
Snehal Joshi,

Roles Data curation, Formal analysis, Visualization, Writing – review & editing

Affiliation Research and Development, Roche Molecular Systems, Inc., Pleasanton, CA, United States of America
⨯
Sidney Scudder,

Roles Formal analysis, Visualization, Writing – review & editing

Current address: Illumina, Foster City, CA, United States of America

Affiliation Research and Development, Roche Molecular Systems, Inc., Pleasanton, CA, United States of America
⨯
Neelima Shrestha,

Roles Visualization, Writing – review & editing

Affiliation Research and Development, Roche Molecular Systems, Inc., Pleasanton, CA, United States of America
⨯
Boe Sorensen,

Roles Visualization, Writing – review & editing

Affiliation Department of Clinical Biochemistry, Aarhus University Hospital, Aarhus, Denmark
⨯
Marilyn Kiral,

Roles Visualization, Writing – review & editing

Current address: Sanofi, Cambridge, MA, United States of America

Affiliation Oncology R&D, Precision Medicine and Biosamples, AstraZeneca, Gaithersburg, MD, United States of America
⨯
Patrick O’Donnell

Roles Conceptualization, Formal analysis, Methodology, Writing – review & editing

Affiliation Research and Development, Roche Molecular Systems, Inc., Pleasanton, CA, United States of America
⨯

Performance characteristics of a polymerase chain reaction-based assay for the detection of EGFR mutations in plasma cell-free DNA from patients with non-small cell lung cancer using cell-free DNA collection tubes

Theresa May,
Michelle S. Clement,
Harkanwal Halait,
Alexander Kohlmann,
Milena Kohlmann,
Jason Lai,
Nitta Lee,
Xiaocheng Li-Sucholeiki,
Peter Meldgaard,
Snehal Joshi

Published: April 9, 2024
https://doi.org/10.1371/journal.pone.0295987

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