Expanding the DNA editing toolbox: Novel lambda integrase variants targeting microalgal and human genome sequences

Jia Wei Siau; Asim Azhar Siddiqui; Sze Yi Lau; Srinivasaraghavan Kannan; Sabrina Peter; Yingying Zeng; Chandra Verma; Peter Droge; John F. Ghadessy

doi:10.1371/journal.pone.0292479

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Open Access

Peer-reviewed

Research Article

Expanding the DNA editing toolbox: Novel lambda integrase variants targeting microalgal and human genome sequences

Jia Wei Siau,

Roles Investigation, Methodology

Affiliation Protein and Peptide Engineering Research Laboratory, Institute of Molecular and Cell Biology, Agency for Science Technology and Research, Singapore, Singapore
⨯
Asim Azhar Siddiqui,

Roles Investigation

Affiliation School of Biological Sciences, Nanyang Technological University, Singapore, Singapore

https://orcid.org/0000-0002-0860-1535

⨯
Sze Yi Lau,

Roles Investigation

Affiliation Protein and Peptide Engineering Research Laboratory, Institute of Molecular and Cell Biology, Agency for Science Technology and Research, Singapore, Singapore
⨯
Srinivasaraghavan Kannan,

Roles Investigation, Writing – original draft

Affiliation Bioinformatics Institute, Agency for Science Technology and Research, Singapore, Singapore
⨯
Sabrina Peter,

Roles Investigation

Affiliation School of Biological Sciences, Nanyang Technological University, Singapore, Singapore
⨯
Yingying Zeng,

Roles Investigation

Affiliation School of Biological Sciences, Nanyang Technological University, Singapore, Singapore
⨯
Chandra Verma,

Roles Formal analysis, Investigation

Affiliation Bioinformatics Institute, Agency for Science Technology and Research, Singapore, Singapore
⨯
Peter Droge,

Roles Conceptualization, Formal analysis, Funding acquisition, Supervision, Writing – original draft, Writing – review & editing

Affiliations School of Biological Sciences, Nanyang Technological University, Singapore, Singapore, LambdaGen Pte. Ltd., Singapore, Singapore
⨯
John F. Ghadessy

Roles Conceptualization, Supervision, Writing – original draft, Writing – review & editing

* E-mail: fghadessy@imcb.a-star.edu.sg

Affiliation Protein and Peptide Engineering Research Laboratory, Institute of Molecular and Cell Biology, Agency for Science Technology and Research, Singapore, Singapore

https://orcid.org/0000-0001-8559-903X

⨯

Expanding the DNA editing toolbox: Novel lambda integrase variants targeting microalgal and human genome sequences

Jia Wei Siau,
Asim Azhar Siddiqui,
Sze Yi Lau,
Srinivasaraghavan Kannan,
Sabrina Peter,
Yingying Zeng,
Chandra Verma,
Peter Droge,
John F. Ghadessy

Published: February 13, 2024
https://doi.org/10.1371/journal.pone.0292479

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Polymerase chain reaction
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Recombination reactions
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Recombinant protein purification
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DNA recombination
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Biochemical simulations
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Recombination-based assay
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