Understanding the biosynthesis of human IgM SAM-6 through a combinatorial expression of mutant subunits that affect product assembly and secretion

Haruki Hasegawa; Songyu Wang; Eddie Kast; Hui-Ting Chou; Mehma Kaur; Tanakorn Janlaor; Mina Mostafavi; Yi-Ling Wang; Peng Li

doi:10.1371/journal.pone.0291568

Loading metrics

Open Access

Peer-reviewed

Research Article

Understanding the biosynthesis of human IgM SAM-6 through a combinatorial expression of mutant subunits that affect product assembly and secretion

Haruki Hasegawa ,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

* E-mail: harukih@amgen.com

Affiliation Discovery Protein Science, Department of Large Molecule Discovery and Research Data Science, Amgen Inc., South San Francisco, CA, United States of America

https://orcid.org/0000-0002-3464-4715

⨯
Songyu Wang,

Roles Data curation, Investigation, Resources, Writing – review & editing

Affiliation Discovery Protein Science, Department of Large Molecule Discovery and Research Data Science, Amgen Inc., South San Francisco, CA, United States of America
⨯
Eddie Kast,

Roles Data curation, Investigation

Affiliation Molecular Analytics, Department of Biologic Therapeutic Discovery, Amgen Inc., South San Francisco, CA, United States of America
⨯
Hui-Ting Chou,

Roles Data curation, Investigation

Affiliation Structural Biology, Department of Small Molecule Therapeutic Discovery, Amgen Inc., South San Francisco, CA, United States of America
⨯
Mehma Kaur,

Roles Resources

Affiliation Discovery Protein Science, Department of Large Molecule Discovery and Research Data Science, Amgen Inc., South San Francisco, CA, United States of America
⨯
Tanakorn Janlaor,

Roles Resources

Affiliation Discovery Protein Science, Department of Large Molecule Discovery and Research Data Science, Amgen Inc., South San Francisco, CA, United States of America

https://orcid.org/0009-0007-1434-3716

⨯
Mina Mostafavi,

Roles Resources

Affiliation Discovery Protein Science, Department of Large Molecule Discovery and Research Data Science, Amgen Inc., South San Francisco, CA, United States of America
⨯
Yi-Ling Wang,

Roles Resources

Affiliation Discovery Protein Science, Department of Large Molecule Discovery and Research Data Science, Amgen Inc., South San Francisco, CA, United States of America
⨯
Peng Li

Roles Resources, Writing – review & editing

Affiliation Discovery Protein Science, Department of Large Molecule Discovery and Research Data Science, Amgen Inc., South San Francisco, CA, United States of America
⨯

Understanding the biosynthesis of human IgM SAM-6 through a combinatorial expression of mutant subunits that affect product assembly and secretion

Haruki Hasegawa,
Songyu Wang,
Eddie Kast,
Hui-Ting Chou,
Mehma Kaur,
Tanakorn Janlaor,
Mina Mostafavi,
Yi-Ling Wang,
Peng Li

Published: June 7, 2024
https://doi.org/10.1371/journal.pone.0291568

Reader Comments (0)

Post a new comment on this article

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

Secretion
Is the Subject Area "Secretion" applicable to this article?

Thanks for your feedback.
Transfection
Is the Subject Area "Transfection" applicable to this article?

Thanks for your feedback.
Disulfide bonds
Is the Subject Area "Disulfide bonds" applicable to this article?

Thanks for your feedback.
Cell cultures
Is the Subject Area "Cell cultures" applicable to this article?

Thanks for your feedback.
Monomers
Is the Subject Area "Monomers" applicable to this article?

Thanks for your feedback.
Point mutation
Is the Subject Area "Point mutation" applicable to this article?

Thanks for your feedback.
Biosynthesis
Is the Subject Area "Biosynthesis" applicable to this article?

Thanks for your feedback.
Solubility
Is the Subject Area "Solubility" applicable to this article?

Thanks for your feedback.