Expansion microscopy using a single anchor molecule for high-yield multiplexed imaging of proteins and RNAs

• Yi Cui ,

  Contributed equally to this work with: Yi Cui, Gaojie Yang

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Software, Validation, Visualization, Writing – original draft, Writing – review & editing

  Current address: NanoString Technologies, Inc., Seattle, Washington, United States of America

  Affiliations McGovern Institute, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, United States of America, Media Arts & Sciences, MIT, Cambridge, Massachusetts, United States of America

  https://orcid.org/0000-0002-8597-0174

  ⨯
• Gaojie Yang ,

  Contributed equally to this work with: Yi Cui, Gaojie Yang

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization

  Affiliations McGovern Institute, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, United States of America, Media Arts & Sciences, MIT, Cambridge, Massachusetts, United States of America

  ⨯
• Daniel R. Goodwin,

  Roles Data curation, Formal analysis, Software

  Affiliations McGovern Institute, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, United States of America, Media Arts & Sciences, MIT, Cambridge, Massachusetts, United States of America

  ⨯
• Ciara H. O’Flanagan,

  Roles Data curation, Investigation

  Affiliation Department of Molecular Oncology, BC Cancer, Vancouver, British Columbia, Canada

  ⨯
• Anubhav Sinha,

  Roles Data curation

  Affiliations McGovern Institute, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, United States of America, Media Arts & Sciences, MIT, Cambridge, Massachusetts, United States of America, Harvard-MIT Program in Health Sciences and Technology, MIT, Cambridge, Massachusetts, United States of America

  https://orcid.org/0000-0002-1545-3163

  ⨯
• Chi Zhang,

  Roles Data curation, Validation

  Affiliations McGovern Institute, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, United States of America, Media Arts & Sciences, MIT, Cambridge, Massachusetts, United States of America

  ⨯
• Kristina E. Kitko,

  Roles Validation

  Current address: Eli Lilly and Company, San Francisco, California, United States of America

  Affiliations McGovern Institute, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, United States of America, Media Arts & Sciences, MIT, Cambridge, Massachusetts, United States of America

  ⨯
• Tay Won Shin,

  Roles Data curation

  Affiliations McGovern Institute, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, United States of America, Media Arts & Sciences, MIT, Cambridge, Massachusetts, United States of America

  https://orcid.org/0000-0002-0061-522X

  ⨯
• Demian Park,

  Roles Resources

  Affiliations McGovern Institute, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, United States of America, Media Arts & Sciences, MIT, Cambridge, Massachusetts, United States of America

  ⨯
• Samuel Aparicio,

  Roles Funding acquisition, Supervision

  Affiliations Department of Molecular Oncology, BC Cancer, Vancouver, British Columbia, Canada, Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada

  ⨯
• CRUK IMAXT Grand Challenge Consortium ,

  ¶List of the IMAXT Consortium investigators is provided in the Acknowledgments.

  ⨯
• Edward S. Boyden

  Roles Conceptualization, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Writing – original draft, Writing – review & editing

  * E-mail: edboyden@mit.edu

  Affiliations McGovern Institute, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, United States of America, Media Arts & Sciences, MIT, Cambridge, Massachusetts, United States of America, Department of Biological Engineering, MIT, Cambridge, Massachusetts, United States of America, Department of Brain and Cognitive Sciences, MIT, Cambridge, Massachusetts, United States of America, Koch Institute for Cancer Research, MIT, Cambridge, Massachusetts, United States of America, Howard Hughes Medical Institute, MIT, Cambridge, Massachusetts, United States of America

  https://orcid.org/0000-0002-0419-3351

  ⨯