Quantitative SARS-CoV-2 subgenomic RNA as a surrogate marker for viral infectivity: Comparison between culture isolation and direct sgRNA quantification

Rossana Scutari; Silvia Renica; Valeria Cento; Alice Nava; Josè Camilla Sammartino; Alessandro Ferrari; Arianna Pani; Marco Merli; Diana Fanti; Chiara Vismara; Francesco Scaglione; Massimo Puoti; Alessandra Bandera; Andrea Gori; Antonio Piralla; Fausto Baldanti; Carlo Federico Perno; Claudia Alteri

doi:10.1371/journal.pone.0291120

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Peer-reviewed

Research Article

Quantitative SARS-CoV-2 subgenomic RNA as a surrogate marker for viral infectivity: Comparison between culture isolation and direct sgRNA quantification

Rossana Scutari ,

Contributed equally to this work with: Rossana Scutari, Silvia Renica

Roles Data curation, Formal analysis, Methodology, Writing – original draft

Affiliations Department of Oncology and Hemato-oncology, University of Milan, Milan, Italy, Multimodal Research Area, Bambino Gesù Children Hospital IRCCS, Rome, Italy
⨯
Silvia Renica ,

Contributed equally to this work with: Rossana Scutari, Silvia Renica

Roles Data curation, Formal analysis, Methodology, Writing – original draft

Affiliation Department of Oncology and Hemato-oncology, University of Milan, Milan, Italy
⨯
Valeria Cento,

Roles Investigation

Affiliations Department of Biomedical Sciences, Humanitas University, Pieve Emanuele, Milan, Italy, IRCSS Humanitas Research Hospital, Rozzano, Milan, Italy
⨯
Alice Nava,

Roles Methodology

Affiliation Chemical-Clinical and Microbiological Analysis, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy
⨯
Josè Camilla Sammartino,

Roles Methodology

Affiliation Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy

https://orcid.org/0000-0003-3707-3118

⨯
Alessandro Ferrari,

Roles Methodology

Affiliation Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy
⨯
Arianna Pani,

Roles Investigation

Affiliation Chemical-Clinical and Microbiological Analysis, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy
⨯
Marco Merli,

Roles Investigation

Affiliation Infectious Diseases Unit, Azienda Socio-Sanitaria Territoriale (ASST) Grande Ospedale Metropolitano Niguarda, Milan, Italy

https://orcid.org/0000-0001-5081-8669

⨯
Diana Fanti,

Roles Investigation

Affiliation Chemical-Clinical and Microbiological Analysis, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy
⨯
Chiara Vismara,

Roles Investigation

Affiliation Chemical-Clinical and Microbiological Analysis, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy
⨯
Francesco Scaglione,

Roles Supervision

Affiliations Department of Oncology and Hemato-oncology, University of Milan, Milan, Italy, Chemical-Clinical and Microbiological Analysis, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy
⨯
Massimo Puoti,

Roles Investigation

Affiliation Infectious Diseases Unit, Azienda Socio-Sanitaria Territoriale (ASST) Grande Ospedale Metropolitano Niguarda, Milan, Italy
⨯
Alessandra Bandera,

Roles Investigation, Supervision

Affiliations Infectious Diseases Unit, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, University of Milan, Milan, Italy, Department of Pathophysiology and Transplantation, University of Milan, Milan, Italy
⨯
Andrea Gori,

Roles Supervision, Writing – review & editing

Affiliations Infectious Diseases Unit, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, University of Milan, Milan, Italy, Department of Pathophysiology and Transplantation, University of Milan, Milan, Italy
⨯
Antonio Piralla,

Roles Conceptualization, Methodology, Writing – review & editing

Affiliation Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy
⨯
Fausto Baldanti,

Roles Conceptualization, Supervision, Writing – review & editing

Affiliations Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy, Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, University of Pavia, Pavia, Italy
⨯
Carlo Federico Perno,

Roles Conceptualization, Supervision, Writing – review & editing

Affiliation Multimodal Research Area, Bambino Gesù Children Hospital IRCCS, Rome, Italy
⨯
[ ... ],
Claudia Alteri

Roles Conceptualization, Data curation, Supervision, Writing – review & editing

* E-mail: claudia.alteri@unimi.it

Affiliations Department of Oncology and Hemato-oncology, University of Milan, Milan, Italy, Multimodal Research Area, Bambino Gesù Children Hospital IRCCS, Rome, Italy

https://orcid.org/0000-0003-4270-5079

⨯
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Quantitative SARS-CoV-2 subgenomic RNA as a surrogate marker for viral infectivity: Comparison between culture isolation and direct sgRNA quantification

Rossana Scutari,
Silvia Renica,
Valeria Cento,
Alice Nava,
Josè Camilla Sammartino,
Alessandro Ferrari,
Arianna Pani,
Marco Merli,
Diana Fanti,
Chiara Vismara

Published: September 1, 2023
https://doi.org/10.1371/journal.pone.0291120

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