Selective DNA-binding of SP120 (rat ortholog of human hnRNP U) is mediated by arginine-glycine rich domain and modulated by RNA

Mary Miyaji; Shinji Kawano; Ryohei Furuta; Emi Murakami; Shogo Ikeda; Kimiko M. Tsutsui; Ken Tsutsui

doi:10.1371/journal.pone.0289599

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Open Access

Peer-reviewed

Research Article

Selective DNA-binding of SP120 (rat ortholog of human hnRNP U) is mediated by arginine-glycine rich domain and modulated by RNA

Mary Miyaji ,

Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Project administration, Validation, Visualization, Writing – original draft

* E-mail: mmiyaji@okayama-u.ac.jp

Affiliation Department of Neurogenomics, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan

https://orcid.org/0000-0003-3968-5994

⨯
Shinji Kawano,

Roles Formal analysis, Investigation, Visualization

Affiliation Faculty of Science, Department of Biochemistry, Okayama University of Science, Okayama, Japan
⨯
Ryohei Furuta,

Roles Investigation, Visualization

Affiliation Department of Neurogenomics, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan
⨯
Emi Murakami,

Roles Investigation

Affiliation Faculty of Science, Department of Biochemistry, Okayama University of Science, Okayama, Japan
⨯
Shogo Ikeda,

Roles Supervision

Affiliation Faculty of Science, Department of Biochemistry, Okayama University of Science, Okayama, Japan
⨯
Kimiko M. Tsutsui,

Roles Conceptualization, Data curation, Formal analysis, Project administration, Supervision, Visualization, Writing – review & editing

Affiliation Department of Neurogenomics, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan
⨯
Ken Tsutsui

Roles Conceptualization, Data curation, Formal analysis, Project administration, Supervision, Visualization, Writing – review & editing

Affiliation Department of Neurogenomics, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan
⨯

Selective DNA-binding of SP120 (rat ortholog of human hnRNP U) is mediated by arginine-glycine rich domain and modulated by RNA

Mary Miyaji,
Shinji Kawano,
Ryohei Furuta,
Emi Murakami,
Shogo Ikeda,
Kimiko M. Tsutsui,
Ken Tsutsui

Published: August 4, 2023
https://doi.org/10.1371/journal.pone.0289599

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DNA-binding proteins
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Arginine
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DNA cloning
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Binding analysis
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Electrophoretic mobility shift assay
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RNA-binding proteins
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Escherichia coli
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Protein domains
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