Optimization of differentiation and transcriptomic profile of THP-1 cells into macrophage by PMA

Tiezhu Liu; Tao Huang; Jiajia Li; Aqian Li; Chuan Li; Xiaoxia Huang; Dexin Li; Shiwen Wang; Mifang Liang

doi:10.1371/journal.pone.0286056

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Open Access

Peer-reviewed

Research Article

Optimization of differentiation and transcriptomic profile of THP-1 cells into macrophage by PMA

Tiezhu Liu ,

Contributed equally to this work with: Tiezhu Liu, Tao Huang

Roles Conceptualization, Formal analysis, Software, Writing – original draft

Affiliation National Health Commission Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China

https://orcid.org/0000-0001-8222-4451

⨯
Tao Huang ,

Contributed equally to this work with: Tiezhu Liu, Tao Huang

Roles Conceptualization, Investigation, Resources

Affiliation National Health Commission Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China
⨯
Jiajia Li,

Roles Formal analysis, Investigation, Software

Affiliation The First Affiliated Hospital of Anhui Medical University, Hefei, China
⨯
Aqian Li,

Roles Supervision, Validation

Affiliation National Health Commission Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China
⨯
Chuan Li,

Roles Project administration

Affiliation National Health Commission Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China
⨯
Xiaoxia Huang,

Roles Conceptualization, Supervision

Affiliation National Health Commission Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China
⨯
Dexin Li,

Roles Methodology, Validation

Affiliation National Health Commission Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China
⨯
Shiwen Wang ,

Roles Funding acquisition, Methodology, Supervision

* E-mail: wangsw@ivdc.chinacdc.cn (SW); mifangl@163.com (ML)

Affiliation National Health Commission Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China
⨯
Mifang Liang

Roles Funding acquisition, Validation, Visualization

* E-mail: wangsw@ivdc.chinacdc.cn (SW); mifangl@163.com (ML)

Affiliation National Health Commission Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China
⨯

Optimization of differentiation and transcriptomic profile of THP-1 cells into macrophage by PMA

Tiezhu Liu,
Tao Huang,
Jiajia Li,
Aqian Li,
Chuan Li,
Xiaoxia Huang,
Dexin Li,
Shiwen Wang,
Mifang Liang

Published: July 17, 2023
https://doi.org/10.1371/journal.pone.0286056

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Cell differentiation
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Macrophages
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RNA sequencing
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Flow cytometry
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Transcriptome analysis
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Cell cycle and cell division
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Gene expression
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Inflammation
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