Isolation of porcine adult cardiomyocytes: Comparison between Langendorff perfusion and tissue slicing-assisted enzyme digestion

Xun Shi; Xiaoli Tang; Fang Yao; Le Wang; Mingzhi Zhang; Xin Wang; Guangxin Yue; Li Wang; Shengshou Hu; Bingying Zhou

doi:10.1371/journal.pone.0285169

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Open Access

Peer-reviewed

Research Article

Isolation of porcine adult cardiomyocytes: Comparison between Langendorff perfusion and tissue slicing-assisted enzyme digestion

Xun Shi,

Roles Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – review & editing

Affiliation State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
⨯
Xiaoli Tang,

Roles Formal analysis, Validation

Affiliation State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
⨯
Fang Yao,

Roles Formal analysis

Affiliation Shenzhen Key Laboratory of Cardiovascular Disease, Fuwai Hospital Chinese Academy of Medical Science, Shenzhen, Shenzhen, China
⨯
Le Wang,

Roles Formal analysis, Validation

Affiliation State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
⨯
Mingzhi Zhang,

Roles Validation

Affiliation State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
⨯
Xin Wang,

Roles Resources

Affiliations Division of Prevention and Community Health, National Center for Cardiovascular Disease, National Clinical Research Center of Cardiovascular Disease, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China, State Key Laboratory of Cardiovascular Disease, Center for Cardiovascular Experimental Study and Evaluation, National Center for Cardiovascular Diseases, Beijing Key Laboratory of Pre-clinical Research and Evaluation for Cardiovascular Implant Materials, Animal Experimental Center, Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College
⨯
Guangxin Yue,

Roles Resources

Affiliation State Key Laboratory of Cardiovascular Disease, Center for Cardiovascular Experimental Study and Evaluation, National Center for Cardiovascular Diseases, Beijing Key Laboratory of Pre-clinical Research and Evaluation for Cardiovascular Implant Materials, Animal Experimental Center, Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College
⨯
Li Wang,

Roles Formal analysis, Software, Supervision

Affiliations State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China, Shenzhen Key Laboratory of Cardiovascular Disease, Fuwai Hospital Chinese Academy of Medical Science, Shenzhen, Shenzhen, China
⨯
Shengshou Hu ,

Roles Conceptualization, Funding acquisition, Supervision

* E-mail: huss@fuwai.pumc.edu.cn (SH); zhouby@fuwai.pumc.edu.cn (BZ)

Affiliations State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China, Shenzhen Key Laboratory of Cardiovascular Disease, Fuwai Hospital Chinese Academy of Medical Science, Shenzhen, Shenzhen, China
⨯
Bingying Zhou

Roles Funding acquisition, Methodology, Project administration, Supervision, Writing – original draft

* E-mail: huss@fuwai.pumc.edu.cn (SH); zhouby@fuwai.pumc.edu.cn (BZ)

Affiliations State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China, Shenzhen Key Laboratory of Cardiovascular Disease, Fuwai Hospital Chinese Academy of Medical Science, Shenzhen, Shenzhen, China

https://orcid.org/0000-0002-9581-5768

⨯

Isolation of porcine adult cardiomyocytes: Comparison between Langendorff perfusion and tissue slicing-assisted enzyme digestion

Xun Shi,
Xiaoli Tang,
Fang Yao,
Le Wang,
Mingzhi Zhang,
Xin Wang,
Guangxin Yue,
Li Wang,
Shengshou Hu,
Bingying Zhou

Published: May 26, 2023
https://doi.org/10.1371/journal.pone.0285169

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Cardiomyocytes
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Action potentials
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Gene expression
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Cell staining
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Adults
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Heart
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Membrane potential
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